Fibrous nanocrystals of hydroxyapatite loaded with TiO2 nanoparticles for the capture and photocatalytic decomposition of specific proteins

Sho Hirakura, Toru Kobayashi, Shohei Ono, Yuya Oaki, Hiroaki Imai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The monomolecular adsorption of lysozyme (LSZ) and bovine serum albumin (BSA) on hydroxyapatite (HAp) was observed by using two types of fibrous crystals elongated in the c-axis. Selective removal of the specific proteins was achieved by the capture and decomposition on the HAp crystals having the particular nanostructures attached with anatase-type TiO2. Bundled short nanorods of ca. 10nm in diameter were suitable for the capture of a relatively small protein molecule, LSZ, due to their high specific surface area, while the adsorption of a relatively large molecule, BSA, was restricted because of the narrow clearance gap between the nanorods. On the other hand, the large protein preferentially adsorbed to long nanoneedles of 30-60nm in diameter, which had a wide gap among the loosely aggregated crystals. The captured proteins were smoothly decomposed with anatase nanoparticles loaded on the fibrous HAp crystals under UV irradiation. Thus, the photocatalytic activity for the decomposition of proteins could be controlled with the adsorption on the surface of the nanostructured HAp crystals.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume79
Issue number1
DOIs
Publication statusPublished - 2010 Aug

Fingerprint

Durapatite
Hydroxyapatite
Nanoparticles
Nanocrystals
nanocrystals
proteins
Decomposition
Proteins
decomposition
nanoparticles
Crystals
Adsorption
Nanotubes
crystals
lysozyme
Muramidase
Bovine Serum Albumin
Nanorods
albumins
anatase

Keywords

  • Adsorption
  • Decomposition
  • Hydroxyapatite
  • Photocatalyst
  • Protein

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Fibrous nanocrystals of hydroxyapatite loaded with TiO2 nanoparticles for the capture and photocatalytic decomposition of specific proteins. / Hirakura, Sho; Kobayashi, Toru; Ono, Shohei; Oaki, Yuya; Imai, Hiroaki.

In: Colloids and Surfaces B: Biointerfaces, Vol. 79, No. 1, 08.2010, p. 131-135.

Research output: Contribution to journalArticle

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