Fictive locomotion induced by octopamine in the earthworm

Kenji Mizutani, Hiroto Ogawa, Junichi Saito, Kotaro Oka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We investigated the function of octopamine (OA) as a motor pattern inducer in the earthworm Eisenia fetida. We used semi-intact preparations, consisting of 20 sequential segmental ganglia of the ventral nerve cord (VNC) together with the body wall from the middle of the animal. Bath-application of 10-4 mol l-1 OA to the semi-intact preparation induced phasic muscle contractions, which are consistent with crawling. In the isolated VNC, OA induced bursts of motor neuron activity in the first lateral nerves. Burst frequency increased with OA concentration, with a large increase in activity observed in the range 10-6-10-4 mol l-1. At 10-4 mol l-1, the coefficient of variation of burst periods (BPs) was smaller than that seen upon application of OA at other concentrations, which is indicative of rhythmic bursts. These rhythmic bursts propagated along the VNC from the anterior to posterior, with a propagation velocity ranging from 60 to 110 mm s-1. This velocity is consistent with the propagation velocity of muscle contraction during crawling behavior in the intact earthworm. From these results, we conclude that fictive crawling motor patterns are observed at 10-4 mol l-1 OA, and that OA can induce rhythmic bursts in the isolated VNC of the earthworm.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalJournal of Experimental Biology
Volume205
Issue number2
Publication statusPublished - 2002

Fingerprint

Octopamine
octopamine
Oligochaeta
locomotion
Locomotion
earthworms
earthworm
ventral nerve cord
contraction
muscle
muscle contraction
Muscle Contraction
animal
Eisenia fetida
Motor Neurons
motor neurons
Baths
integument
Ganglia
nerve tissue

Keywords

  • Central pattern generator
  • Crawling
  • Earthworm
  • Eisenia fetida
  • Fictive locomotion
  • Motor pattern
  • Octopamine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Fictive locomotion induced by octopamine in the earthworm. / Mizutani, Kenji; Ogawa, Hiroto; Saito, Junichi; Oka, Kotaro.

In: Journal of Experimental Biology, Vol. 205, No. 2, 2002, p. 265-271.

Research output: Contribution to journalArticle

Mizutani, K, Ogawa, H, Saito, J & Oka, K 2002, 'Fictive locomotion induced by octopamine in the earthworm', Journal of Experimental Biology, vol. 205, no. 2, pp. 265-271.
Mizutani, Kenji ; Ogawa, Hiroto ; Saito, Junichi ; Oka, Kotaro. / Fictive locomotion induced by octopamine in the earthworm. In: Journal of Experimental Biology. 2002 ; Vol. 205, No. 2. pp. 265-271.
@article{80d96eacd7454c109b9aecf66ddc0005,
title = "Fictive locomotion induced by octopamine in the earthworm",
abstract = "We investigated the function of octopamine (OA) as a motor pattern inducer in the earthworm Eisenia fetida. We used semi-intact preparations, consisting of 20 sequential segmental ganglia of the ventral nerve cord (VNC) together with the body wall from the middle of the animal. Bath-application of 10-4 mol l-1 OA to the semi-intact preparation induced phasic muscle contractions, which are consistent with crawling. In the isolated VNC, OA induced bursts of motor neuron activity in the first lateral nerves. Burst frequency increased with OA concentration, with a large increase in activity observed in the range 10-6-10-4 mol l-1. At 10-4 mol l-1, the coefficient of variation of burst periods (BPs) was smaller than that seen upon application of OA at other concentrations, which is indicative of rhythmic bursts. These rhythmic bursts propagated along the VNC from the anterior to posterior, with a propagation velocity ranging from 60 to 110 mm s-1. This velocity is consistent with the propagation velocity of muscle contraction during crawling behavior in the intact earthworm. From these results, we conclude that fictive crawling motor patterns are observed at 10-4 mol l-1 OA, and that OA can induce rhythmic bursts in the isolated VNC of the earthworm.",
keywords = "Central pattern generator, Crawling, Earthworm, Eisenia fetida, Fictive locomotion, Motor pattern, Octopamine",
author = "Kenji Mizutani and Hiroto Ogawa and Junichi Saito and Kotaro Oka",
year = "2002",
language = "English",
volume = "205",
pages = "265--271",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "2",

}

TY - JOUR

T1 - Fictive locomotion induced by octopamine in the earthworm

AU - Mizutani, Kenji

AU - Ogawa, Hiroto

AU - Saito, Junichi

AU - Oka, Kotaro

PY - 2002

Y1 - 2002

N2 - We investigated the function of octopamine (OA) as a motor pattern inducer in the earthworm Eisenia fetida. We used semi-intact preparations, consisting of 20 sequential segmental ganglia of the ventral nerve cord (VNC) together with the body wall from the middle of the animal. Bath-application of 10-4 mol l-1 OA to the semi-intact preparation induced phasic muscle contractions, which are consistent with crawling. In the isolated VNC, OA induced bursts of motor neuron activity in the first lateral nerves. Burst frequency increased with OA concentration, with a large increase in activity observed in the range 10-6-10-4 mol l-1. At 10-4 mol l-1, the coefficient of variation of burst periods (BPs) was smaller than that seen upon application of OA at other concentrations, which is indicative of rhythmic bursts. These rhythmic bursts propagated along the VNC from the anterior to posterior, with a propagation velocity ranging from 60 to 110 mm s-1. This velocity is consistent with the propagation velocity of muscle contraction during crawling behavior in the intact earthworm. From these results, we conclude that fictive crawling motor patterns are observed at 10-4 mol l-1 OA, and that OA can induce rhythmic bursts in the isolated VNC of the earthworm.

AB - We investigated the function of octopamine (OA) as a motor pattern inducer in the earthworm Eisenia fetida. We used semi-intact preparations, consisting of 20 sequential segmental ganglia of the ventral nerve cord (VNC) together with the body wall from the middle of the animal. Bath-application of 10-4 mol l-1 OA to the semi-intact preparation induced phasic muscle contractions, which are consistent with crawling. In the isolated VNC, OA induced bursts of motor neuron activity in the first lateral nerves. Burst frequency increased with OA concentration, with a large increase in activity observed in the range 10-6-10-4 mol l-1. At 10-4 mol l-1, the coefficient of variation of burst periods (BPs) was smaller than that seen upon application of OA at other concentrations, which is indicative of rhythmic bursts. These rhythmic bursts propagated along the VNC from the anterior to posterior, with a propagation velocity ranging from 60 to 110 mm s-1. This velocity is consistent with the propagation velocity of muscle contraction during crawling behavior in the intact earthworm. From these results, we conclude that fictive crawling motor patterns are observed at 10-4 mol l-1 OA, and that OA can induce rhythmic bursts in the isolated VNC of the earthworm.

KW - Central pattern generator

KW - Crawling

KW - Earthworm

KW - Eisenia fetida

KW - Fictive locomotion

KW - Motor pattern

KW - Octopamine

UR - http://www.scopus.com/inward/record.url?scp=0036330411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036330411&partnerID=8YFLogxK

M3 - Article

C2 - 11821492

AN - SCOPUS:0036330411

VL - 205

SP - 265

EP - 271

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 2

ER -