Fixation-Free Evaluation of Cardiac Contractile Force by Human iPSC-Derived Cardiac Core-Shell Microfiber

Akari Masuda, Keisuke Fukada, Shun Itai, Yuta Kurashina, Shuichi Akizuki, Shugo Tohyama, Jun Fujita, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose a core-shell microfiber-shaped cardiac tissue as a drug screening model with a fixation-free contractile force measurement system. The contractile force of the hiPSC-derived cardiac tissue can be calculated only with a motion analysis, thanks to a shell of hydrogel covering the cardiac tissue. Thus, the system can evaluate the behavior of the tissue in drug screenings without any specific equipment. We demonstrated drug screening tests with pharmaceuticals on our microfiber tissues, resulting in 102.7% increase of contractile force with isoproterenol and 56.3% decrease with propranolol. Our cardiac drug screening model can easily reproduce and evaluate cardiac disease models, and would contribute to the pathological research of unexplained cardiac diseases and pharmacokinetic testing.

Original languageEnglish
Title of host publication35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
PublisherIEEE Computer Society
Pages172-175
Number of pages4
ISBN (Electronic)9781665409117
DOIs
Publication statusPublished - 2022
Event35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan
Duration: 2022 Jan 92022 Jan 13

Publication series

NameIEEE Symposium on Mass Storage Systems and Technologies
Volume2022-January
ISSN (Print)2160-1968

Conference

Conference35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Country/TerritoryJapan
CityTokyo
Period22/1/922/1/13

Keywords

  • Cardiac Tissue
  • Hydrogel
  • Microfiber
  • Microfluidics
  • Tissue Engineering
  • iPSCs

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'Fixation-Free Evaluation of Cardiac Contractile Force by Human iPSC-Derived Cardiac Core-Shell Microfiber'. Together they form a unique fingerprint.

Cite this