Flagella facilitate escape of Salmonella from oncotic macrophages

Gen Ichiro Sano, Yasunari Takada, Shinichi Goto, Kenta Maruyama, Yutaka Shindo, Kotaro Oka, Hidenori Matsui, Koichi Matsuo

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Abstract

The intracellular parasite Salmonella enterica serovar Typhimurium causes a typhoid-like systemic disease in mice. Whereas the survival of Salmonella in phagocytes is well understood, little has been documented about the exit of intracellular Salmonella from host cells. Here we report that in a population of infected macrophages Salmonella induces "oncosis," an irreversible progression to eukaryotic cell death characterized by swelling of the entire cell body. Oncotic macrophages (OnMφs) are terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling negative and lack actin filaments (F-actin). The plasma membrane of OnMφs filled with bacilli remains impermeable, and intracellular Salmonella bacilli move vigorously using flagella. Eventually, intracellular Salmonella bacilli intermittently exit host cells in a flagellum-dependent manner. These results suggest that induction of macrophage oncosis and intracellular accumulation of flagellated bacilli constitute a strategy whereby Salmonella escapes from host macrophages.

Original languageEnglish
Pages (from-to)8224-8232
Number of pages9
JournalJournal of Bacteriology
Volume189
Issue number22
DOIs
Publication statusPublished - 2007 Nov 1

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Sano, G. I., Takada, Y., Goto, S., Maruyama, K., Shindo, Y., Oka, K., Matsui, H., & Matsuo, K. (2007). Flagella facilitate escape of Salmonella from oncotic macrophages. Journal of Bacteriology, 189(22), 8224-8232. https://doi.org/10.1128/JB.00898-07