Flagella facilitate escape of Salmonella from oncotic macrophages

Gen Ichiro Sano, Yasunari Takada, Shinichi Goto, Kenta Maruyama, Yutaka Shindo, Kotaro Oka, Hidenori Matsui, Koichi Matsuo

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The intracellular parasite Salmonella enterica serovar Typhimurium causes a typhoid-like systemic disease in mice. Whereas the survival of Salmonella in phagocytes is well understood, little has been documented about the exit of intracellular Salmonella from host cells. Here we report that in a population of infected macrophages Salmonella induces "oncosis," an irreversible progression to eukaryotic cell death characterized by swelling of the entire cell body. Oncotic macrophages (OnMφs) are terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling negative and lack actin filaments (F-actin). The plasma membrane of OnMφs filled with bacilli remains impermeable, and intracellular Salmonella bacilli move vigorously using flagella. Eventually, intracellular Salmonella bacilli intermittently exit host cells in a flagellum-dependent manner. These results suggest that induction of macrophage oncosis and intracellular accumulation of flagellated bacilli constitute a strategy whereby Salmonella escapes from host macrophages.

Original languageEnglish
Pages (from-to)8224-8232
Number of pages9
JournalJournal of Bacteriology
Volume189
Issue number22
DOIs
Publication statusPublished - 2007 Nov

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Flagella
Salmonella
Macrophages
Bacillus
Salmonella enterica
DNA Nucleotidylexotransferase
Typhoid Fever
Eukaryotic Cells
Phagocytes
Biotin
Actin Cytoskeleton
Actins
Parasites
Cell Death
Cell Membrane
Population

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Flagella facilitate escape of Salmonella from oncotic macrophages. / Sano, Gen Ichiro; Takada, Yasunari; Goto, Shinichi; Maruyama, Kenta; Shindo, Yutaka; Oka, Kotaro; Matsui, Hidenori; Matsuo, Koichi.

In: Journal of Bacteriology, Vol. 189, No. 22, 11.2007, p. 8224-8232.

Research output: Contribution to journalArticle

Sano, GI, Takada, Y, Goto, S, Maruyama, K, Shindo, Y, Oka, K, Matsui, H & Matsuo, K 2007, 'Flagella facilitate escape of Salmonella from oncotic macrophages', Journal of Bacteriology, vol. 189, no. 22, pp. 8224-8232. https://doi.org/10.1128/JB.00898-07
Sano GI, Takada Y, Goto S, Maruyama K, Shindo Y, Oka K et al. Flagella facilitate escape of Salmonella from oncotic macrophages. Journal of Bacteriology. 2007 Nov;189(22):8224-8232. https://doi.org/10.1128/JB.00898-07
Sano, Gen Ichiro ; Takada, Yasunari ; Goto, Shinichi ; Maruyama, Kenta ; Shindo, Yutaka ; Oka, Kotaro ; Matsui, Hidenori ; Matsuo, Koichi. / Flagella facilitate escape of Salmonella from oncotic macrophages. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 22. pp. 8224-8232.
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