Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells

Atsushi Yoshioka, Ryuichi Okamoto, Shigeru Oshima, Junko Akiyama, Kiichiro Tsuchiya, Tetsuya Nakamura, Takanori Kanai, Mamoru Watanabe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

IL-7 is a cytokine, which regulates development, maintenance and proliferation of T lymphocytes within the human immune system. Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close contact with numerous bacterial stimuli also produce IL-7. Here we show that secretion of IL-7 by IECs is significantly suppressed upon stimulation by various bacterial components, including flagellin. Analysis of the intracellular mechanism by which flagellin regulates IL-7 production revealed that flagellin down-regulates expression of the two major transcripts encoding IL-7. Surprisingly, such function of flagellin was independent from the known transcriptional regulation of the IL-7 gene, as no significant change was observed in the transcriptional activity regulated by the previously identified promoter region. As the stability of IL-7 mRNA also remained unchanged upon flagellin stimulation, results suggested the possible involvement of a yet unknown transcriptional regulation of the IL-7 gene. These results describe a novel regulation of IL-7 production by bacterial stimuli, presumably mediated via Toll-like receptors. The present system might contribute to regulate the local lymphocyte pool, in response to the gut luminal or sub-mucosal bacterial abundance.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalCytokine
Volume44
Issue number1
DOIs
Publication statusPublished - 2008 Oct
Externally publishedYes

Fingerprint

Intestinal Secretions
Flagellin
Interleukin-7
Epithelial Cells
Genes
Thymus
T-cells
Lymphocytes
Immune system
Toll-Like Receptors
Genetic Promoter Regions
Thymus Gland
Immune System
Bone
Down-Regulation
Bone Marrow
Maintenance
Cytokines

Keywords

  • Flagellin
  • IL-7
  • Intestinal epithelial cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Yoshioka, A., Okamoto, R., Oshima, S., Akiyama, J., Tsuchiya, K., Nakamura, T., ... Watanabe, M. (2008). Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells. Cytokine, 44(1), 57-64. https://doi.org/10.1016/j.cyto.2008.06.004

Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells. / Yoshioka, Atsushi; Okamoto, Ryuichi; Oshima, Shigeru; Akiyama, Junko; Tsuchiya, Kiichiro; Nakamura, Tetsuya; Kanai, Takanori; Watanabe, Mamoru.

In: Cytokine, Vol. 44, No. 1, 10.2008, p. 57-64.

Research output: Contribution to journalArticle

Yoshioka, A, Okamoto, R, Oshima, S, Akiyama, J, Tsuchiya, K, Nakamura, T, Kanai, T & Watanabe, M 2008, 'Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells', Cytokine, vol. 44, no. 1, pp. 57-64. https://doi.org/10.1016/j.cyto.2008.06.004
Yoshioka A, Okamoto R, Oshima S, Akiyama J, Tsuchiya K, Nakamura T et al. Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells. Cytokine. 2008 Oct;44(1):57-64. https://doi.org/10.1016/j.cyto.2008.06.004
Yoshioka, Atsushi ; Okamoto, Ryuichi ; Oshima, Shigeru ; Akiyama, Junko ; Tsuchiya, Kiichiro ; Nakamura, Tetsuya ; Kanai, Takanori ; Watanabe, Mamoru. / Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells. In: Cytokine. 2008 ; Vol. 44, No. 1. pp. 57-64.
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