Flow cytometric analysis of surface antigens on human conjunctival epithelial cells

Tsutomu Fujihara, Tsutomu Takeuchi, Keiko Saito, Kazuo Tsubota

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β1 (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β2 integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalOphthalmic research
Volume29
Issue number2
DOIs
Publication statusPublished - 1997 Jan 1
Externally publishedYes

Keywords

  • Adhesion molecule
  • Brush cytology
  • Conjunctival epithelial cells
  • Flow cytometry
  • Integrin

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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