Flow cytometric analysis of surface antigens on human conjunctival epithelial cells

Tsutomu Fujihara; Tsutomu Takeuchi; Keiko Saito; Kazuo Tsubota

doi:10.1159/000268003

Flow cytometric analysis of surface antigens on human conjunctival epithelial cells

Tsutomu Fujihara, Tsutomu Takeuchi, Keiko Saito, Kazuo Tsubota

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β₁ (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β₂ integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.

Original language	English
Pages (from-to)	103-109
Number of pages	7
Journal	Ophthalmic research
Volume	29
Issue number	2
DOIs	https://doi.org/10.1159/000268003
Publication status	Published - 1997 Jan 1
Externally published	Yes

Keywords

Adhesion molecule
Brush cytology
Conjunctival epithelial cells
Flow cytometry
Integrin

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000268003

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title = "Flow cytometric analysis of surface antigens on human conjunctival epithelial cells",

abstract = "We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β1 (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β2 integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.",

keywords = "Adhesion molecule, Brush cytology, Conjunctival epithelial cells, Flow cytometry, Integrin",

author = "Tsutomu Fujihara and Tsutomu Takeuchi and Keiko Saito and Kazuo Tsubota",

year = "1997",

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doi = "10.1159/000268003",

language = "English",

volume = "29",

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journal = "Ophthalmic Research",

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T1 - Flow cytometric analysis of surface antigens on human conjunctival epithelial cells

AU - Fujihara, Tsutomu

AU - Takeuchi, Tsutomu

AU - Saito, Keiko

AU - Tsubota, Kazuo

PY - 1997/1/1

Y1 - 1997/1/1

N2 - We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β1 (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β2 integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.

AB - We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β1 (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β2 integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.

KW - Adhesion molecule

KW - Brush cytology

KW - Conjunctival epithelial cells

KW - Flow cytometry

KW - Integrin

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AN - SCOPUS:0030988485

SN - 0030-3747

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JO - Ophthalmic Research

JF - Ophthalmic Research

IS - 2

ER -

Flow cytometric analysis of surface antigens on human conjunctival epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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