We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β1 (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β2 integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.
- Adhesion molecule
- Brush cytology
- Conjunctival epithelial cells
- Flow cytometry
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience