Flow-independent myocardial ischemia induced by endothelin-1: An NADH fluorescence analysis

Soushin Inoue, Shingo Hori, Takeshi Adachi, Koji Miyazaki, Shingo Kyotani, Keiichi Fukuda, Hidezo Mori, Hiroe Nakazawa, Naoki Aikawa, Satoshi Ogawa

Research output: Contribution to journalArticle

Abstract

The endothelin-1 (ET-1) is known to cause myocardial ischemia; however, whether this effect is entirely dependent on vasoconstriction is uncertain. The aim of this study was to characterize the myocardial ischemia after the intracoronary administration of endothelin-1, and compare it with that induced by coronary stenosis.In the left anterior descending coronary artery of 15 dogs, a mild inflow reduction (30%) was produced for 10 minutes using intracoronary endothelin-1 (46 ± 33 pmol/min) or coronary stenosis. The hearts were rapidly cross-sectioned at short axial plane and freeze-clamped within 120 milliseconds using a specially developed device to visualize and quantify the area of ischemia (%IA) with NADH fluorescence photography. The %IA was larger in the endothelin-1 group than in the stenosis group (66 ± 23 versus 18 ± 18, P = 0.0005); furthermore, the ischemia was transmural in the ET-1 group, but limited to subendocardium in the stenosis group. ET-1 increased the coronary arterial resistance especially in subepicardial region and produced smaller ischemic foci in microcirculation. The mechanism of larger ischemia produced by ET-1 might depend on pro-ischemic effects on myocytes and vasoconstriction of the coronary microcirculation, predominantly in the subepicardium in vivo.

Original languageEnglish
Pages (from-to)810-816
Number of pages7
JournalJournal of Cardiovascular Pharmacology
Volume46
Issue number6
DOIs
Publication statusPublished - 2005 Dec

Fingerprint

Endothelin-1
NAD
Myocardial Ischemia
Fluorescence
Ischemia
Coronary Stenosis
Microcirculation
Vasoconstriction
Pathologic Constriction
Photography
Muscle Cells
Coronary Vessels
Dogs
Equipment and Supplies

Keywords

  • Coronary circulation
  • Endothelin-1
  • Microcirculation
  • Myocardial ischemia
  • NADH fluorescence
  • Pro-ischemia

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Flow-independent myocardial ischemia induced by endothelin-1 : An NADH fluorescence analysis. / Inoue, Soushin; Hori, Shingo; Adachi, Takeshi; Miyazaki, Koji; Kyotani, Shingo; Fukuda, Keiichi; Mori, Hidezo; Nakazawa, Hiroe; Aikawa, Naoki; Ogawa, Satoshi.

In: Journal of Cardiovascular Pharmacology, Vol. 46, No. 6, 12.2005, p. 810-816.

Research output: Contribution to journalArticle

Inoue, S, Hori, S, Adachi, T, Miyazaki, K, Kyotani, S, Fukuda, K, Mori, H, Nakazawa, H, Aikawa, N & Ogawa, S 2005, 'Flow-independent myocardial ischemia induced by endothelin-1: An NADH fluorescence analysis', Journal of Cardiovascular Pharmacology, vol. 46, no. 6, pp. 810-816. https://doi.org/10.1097/01.fjc.0000188280.03123.60
Inoue, Soushin ; Hori, Shingo ; Adachi, Takeshi ; Miyazaki, Koji ; Kyotani, Shingo ; Fukuda, Keiichi ; Mori, Hidezo ; Nakazawa, Hiroe ; Aikawa, Naoki ; Ogawa, Satoshi. / Flow-independent myocardial ischemia induced by endothelin-1 : An NADH fluorescence analysis. In: Journal of Cardiovascular Pharmacology. 2005 ; Vol. 46, No. 6. pp. 810-816.
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