Abstract
The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.
| Original language | English |
|---|---|
| Pages (from-to) | 297-310 |
| Number of pages | 14 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2004 |
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Keywords
- Flow shear stress
- K-ras codon 12
- Point mutation
- Polymer microsphere
- Sandwich method
- Surface plasmon resonance (SPR)
ASJC Scopus subject areas
- Biophysics
Cite this
Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance. / Sato, Yasunobu; Sato, Yuka; Okumura, Aya; Suzuki, Koji; Kawaguchi, Haruma.
In: Journal of Biomaterials Science, Polymer Edition, Vol. 15, No. 3, 2004, p. 297-310.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance
AU - Sato, Yasunobu
AU - Sato, Yuka
AU - Okumura, Aya
AU - Suzuki, Koji
AU - Kawaguchi, Haruma
PY - 2004
Y1 - 2004
N2 - The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.
AB - The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.
KW - Flow shear stress
KW - K-ras codon 12
KW - Point mutation
KW - Polymer microsphere
KW - Sandwich method
KW - Surface plasmon resonance (SPR)
UR - http://www.scopus.com/inward/record.url?scp=1842780876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842780876&partnerID=8YFLogxK
U2 - 10.1163/156856204322977193
DO - 10.1163/156856204322977193
M3 - Article
C2 - 15147163
AN - SCOPUS:1842780876
VL - 15
SP - 297
EP - 310
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 3
ER -