Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance

Yasunobu Sato, Yuka Sato, Aya Okumura, Koji Suzuki, Haruma Kawaguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.

Original languageEnglish
Pages (from-to)297-310
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume15
Issue number3
DOIs
Publication statusPublished - 2004

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Microspheres
Plastic flow
Point Mutation
Polymers
DNA
Biosensing Techniques
Biosensors
Single-Stranded DNA
Viscosity
Gold
Glycerol
Limit of Detection
Shear stress
Buffers
Flow rate
Equipment and Supplies

Keywords

  • Flow shear stress
  • K-ras codon 12
  • Point mutation
  • Polymer microsphere
  • Sandwich method
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • Biophysics

Cite this

Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance. / Sato, Yasunobu; Sato, Yuka; Okumura, Aya; Suzuki, Koji; Kawaguchi, Haruma.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 15, No. 3, 2004, p. 297-310.

Research output: Contribution to journalArticle

Sato, Yasunobu ; Sato, Yuka ; Okumura, Aya ; Suzuki, Koji ; Kawaguchi, Haruma. / Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance. In: Journal of Biomaterials Science, Polymer Edition. 2004 ; Vol. 15, No. 3. pp. 297-310.
@article{44e38c1569f741cdad1ce04a82430ffc,
title = "Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance",
abstract = "The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.",
keywords = "Flow shear stress, K-ras codon 12, Point mutation, Polymer microsphere, Sandwich method, Surface plasmon resonance (SPR)",
author = "Yasunobu Sato and Yuka Sato and Aya Okumura and Koji Suzuki and Haruma Kawaguchi",
year = "2004",
doi = "10.1163/156856204322977193",
language = "English",
volume = "15",
pages = "297--310",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "3",

}

TY - JOUR

T1 - Flow-stress-induced discrimination of a K-ras point mutation by sandwiched polymer microsphere-enhanced surface plasmon resonance

AU - Sato, Yasunobu

AU - Sato, Yuka

AU - Okumura, Aya

AU - Suzuki, Koji

AU - Kawaguchi, Haruma

PY - 2004

Y1 - 2004

N2 - The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.

AB - The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.

KW - Flow shear stress

KW - K-ras codon 12

KW - Point mutation

KW - Polymer microsphere

KW - Sandwich method

KW - Surface plasmon resonance (SPR)

UR - http://www.scopus.com/inward/record.url?scp=1842780876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842780876&partnerID=8YFLogxK

U2 - 10.1163/156856204322977193

DO - 10.1163/156856204322977193

M3 - Article

C2 - 15147163

AN - SCOPUS:1842780876

VL - 15

SP - 297

EP - 310

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 3

ER -