Abstract
The highly sensitive detection of a K-ras point mutation with the aid of DNA-carrying microspheres as a flow-stress receptor is proposed at the surface of a surface plasmon resonance (SPR) biosensor. Single-stranded DNAs were immobilized onto epoxy-group-derivatized gold surfaces and the hybridization of DNA targets was monitored. The subsequent interaction with DNA-carrying micospheres enhanced the SPR response. The increase of flow rate during the event of dissociation changed the amount of detachment of the DNA-carrying microspheres for the mismatched pair. In addition, the viscosity was changed by addition of glycerol to the buffer. The increase of shear stress from the flow resulted in detachment of DNA-carrying microspheres hybridized with the mismatched sequence and increased the ability to discriminate a point mutation. This is a new method which not only increases the lower detection limit of evanescent wave-based biosensors, but also the ability to discriminate a point mutation which is a critical factor for ultrasensitive DNA detection in flow devices.
Original language | English |
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Pages (from-to) | 297-310 |
Number of pages | 14 |
Journal | Journal of Biomaterials Science, Polymer Edition |
Volume | 15 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2004 |
Keywords
- Flow shear stress
- K-ras codon 12
- Point mutation
- Polymer microsphere
- Sandwich method
- Surface plasmon resonance (SPR)
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering