Fluorescence analysis for thermo‐sensitive hydrogel microspheres

K. Fujimoto, Y. Nakajima, M. Kashiwabara, H. Kawaguchi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Poly(N‐isopropylacrylamide) (PNIPAM) hydrogel microspheres were prepared by precipitation polymerization above the polymer's lower critical solution temperature (LCST) in water. The hydrodynamic size of PNIPAM microspheres exhibited a thermo‐sensitive change around the LCST. In order to obtain information about the interface of the hydrogels, contact angle measurement, protein adsorption, and fluorescence analysis were performed. Contact angle measurement and protein adsorption showed a gradual change around the LCST similar to the change of the hydrodynamic size. However, fluorescence study exhibited a sharp change at the LCST. In addition, a quenching experiment was employed to elucidate the location of the fluorescent probes. In the PNIPAM latex, the probes were located where they were less quenchable as compared to the PNIPAM solution. It seems probable that the probes can penetrate into the crosslinked PNIPAM hydrogel and might reflect the inner environment of the hydrogel. The effect of salt on thermo‐sensitive behaviour was also detected by fluorescence analysis. On the other hand, the hydrogel microsphere prepared from poly(acryloyl pyrrolidine) (PAPr) showed a gradual change in fluorescence with temperature.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalPolymer International
Volume30
Issue number2
DOIs
Publication statusPublished - 1993

Keywords

  • fluorescence
  • hydrogel
  • hydrophobic probe
  • interface
  • microsphere
  • quenching
  • thermo‐sensitive

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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