Fluorescence analysis for thermosensitive hydrogel microspheres

Keiji Fujimoto, Y. Nakajima, M. Kashiwabara, H. Kawaguchi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) (PNIPAM) hydrogel microspheres were prepared by precipitation polymerization above the polymer's lower critical solution temperature (LCST) in water. The hydrodynamic size of PNIPAM microspheres exhibited a thermo-sensitive change around the LCST. In order to obtain information about the interface of the hydrogels, contact angle measurement, protein adsorption, and fluorescence analysis were performed. Contact angle measurement and protein adsorption showed a gradual change around the LCST similar to the change of the hydrodynamic size. However, fluorescence study exhibited a sharp change at the LCST. In addition, a quenching experiment was employed to elucidate the location of the fluorescent probes. In the PNIPAM latex, the probes were located where they were less quenchable as compared to the PNIPAM solution. It seems probable that the probes can penetrate into the crosslinked PNIPAM hydrogel and might reflect the inner environment of the hydrogel. The effect of salt on thermo-sensitive behaviour was also detected by fluorescence analysis. On the other hand, the hydrogel microsphere prepared from poly(acryloyl pyrrolidine) (PAPr) showed a gradual change in fluorescence with temperature.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalPolymer International
Volume30
Issue number2
Publication statusPublished - 1993

Fingerprint

Hydrogel
Microspheres
Hydrogels
Fluorescence
Angle measurement
Contact angle
Hydrodynamics
Temperature
Proteins
Adsorption
Latex
Latexes
Fluorescent Dyes
Quenching
Polymers
Salts
Polymerization
poly-N-isopropylacrylamide
Water
Experiments

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

Fujimoto, K., Nakajima, Y., Kashiwabara, M., & Kawaguchi, H. (1993). Fluorescence analysis for thermosensitive hydrogel microspheres. Polymer International, 30(2), 237-241.

Fluorescence analysis for thermosensitive hydrogel microspheres. / Fujimoto, Keiji; Nakajima, Y.; Kashiwabara, M.; Kawaguchi, H.

In: Polymer International, Vol. 30, No. 2, 1993, p. 237-241.

Research output: Contribution to journalArticle

Fujimoto, K, Nakajima, Y, Kashiwabara, M & Kawaguchi, H 1993, 'Fluorescence analysis for thermosensitive hydrogel microspheres', Polymer International, vol. 30, no. 2, pp. 237-241.
Fujimoto K, Nakajima Y, Kashiwabara M, Kawaguchi H. Fluorescence analysis for thermosensitive hydrogel microspheres. Polymer International. 1993;30(2):237-241.
Fujimoto, Keiji ; Nakajima, Y. ; Kashiwabara, M. ; Kawaguchi, H. / Fluorescence analysis for thermosensitive hydrogel microspheres. In: Polymer International. 1993 ; Vol. 30, No. 2. pp. 237-241.
@article{f76db7671cef4c5c8adf4b66a00b8871,
title = "Fluorescence analysis for thermosensitive hydrogel microspheres",
abstract = "Poly(N-isopropylacrylamide) (PNIPAM) hydrogel microspheres were prepared by precipitation polymerization above the polymer's lower critical solution temperature (LCST) in water. The hydrodynamic size of PNIPAM microspheres exhibited a thermo-sensitive change around the LCST. In order to obtain information about the interface of the hydrogels, contact angle measurement, protein adsorption, and fluorescence analysis were performed. Contact angle measurement and protein adsorption showed a gradual change around the LCST similar to the change of the hydrodynamic size. However, fluorescence study exhibited a sharp change at the LCST. In addition, a quenching experiment was employed to elucidate the location of the fluorescent probes. In the PNIPAM latex, the probes were located where they were less quenchable as compared to the PNIPAM solution. It seems probable that the probes can penetrate into the crosslinked PNIPAM hydrogel and might reflect the inner environment of the hydrogel. The effect of salt on thermo-sensitive behaviour was also detected by fluorescence analysis. On the other hand, the hydrogel microsphere prepared from poly(acryloyl pyrrolidine) (PAPr) showed a gradual change in fluorescence with temperature.",
author = "Keiji Fujimoto and Y. Nakajima and M. Kashiwabara and H. Kawaguchi",
year = "1993",
language = "English",
volume = "30",
pages = "237--241",
journal = "Polymer International",
issn = "0959-8103",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Fluorescence analysis for thermosensitive hydrogel microspheres

AU - Fujimoto, Keiji

AU - Nakajima, Y.

AU - Kashiwabara, M.

AU - Kawaguchi, H.

PY - 1993

Y1 - 1993

N2 - Poly(N-isopropylacrylamide) (PNIPAM) hydrogel microspheres were prepared by precipitation polymerization above the polymer's lower critical solution temperature (LCST) in water. The hydrodynamic size of PNIPAM microspheres exhibited a thermo-sensitive change around the LCST. In order to obtain information about the interface of the hydrogels, contact angle measurement, protein adsorption, and fluorescence analysis were performed. Contact angle measurement and protein adsorption showed a gradual change around the LCST similar to the change of the hydrodynamic size. However, fluorescence study exhibited a sharp change at the LCST. In addition, a quenching experiment was employed to elucidate the location of the fluorescent probes. In the PNIPAM latex, the probes were located where they were less quenchable as compared to the PNIPAM solution. It seems probable that the probes can penetrate into the crosslinked PNIPAM hydrogel and might reflect the inner environment of the hydrogel. The effect of salt on thermo-sensitive behaviour was also detected by fluorescence analysis. On the other hand, the hydrogel microsphere prepared from poly(acryloyl pyrrolidine) (PAPr) showed a gradual change in fluorescence with temperature.

AB - Poly(N-isopropylacrylamide) (PNIPAM) hydrogel microspheres were prepared by precipitation polymerization above the polymer's lower critical solution temperature (LCST) in water. The hydrodynamic size of PNIPAM microspheres exhibited a thermo-sensitive change around the LCST. In order to obtain information about the interface of the hydrogels, contact angle measurement, protein adsorption, and fluorescence analysis were performed. Contact angle measurement and protein adsorption showed a gradual change around the LCST similar to the change of the hydrodynamic size. However, fluorescence study exhibited a sharp change at the LCST. In addition, a quenching experiment was employed to elucidate the location of the fluorescent probes. In the PNIPAM latex, the probes were located where they were less quenchable as compared to the PNIPAM solution. It seems probable that the probes can penetrate into the crosslinked PNIPAM hydrogel and might reflect the inner environment of the hydrogel. The effect of salt on thermo-sensitive behaviour was also detected by fluorescence analysis. On the other hand, the hydrogel microsphere prepared from poly(acryloyl pyrrolidine) (PAPr) showed a gradual change in fluorescence with temperature.

UR - http://www.scopus.com/inward/record.url?scp=0027168016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027168016&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 237

EP - 241

JO - Polymer International

JF - Polymer International

SN - 0959-8103

IS - 2

ER -