Fluorescence detection of serum albumin with a turnover-based sensor utilizing Kemp elimination reaction

Shingo Sakamoto, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The Kemp elimination reaction is a well-known chemical reaction that is facilitated on a protein surface microenvironment, and in particular is highly accelerated in a unique binding pocket of serum albumin. We have designed and synthesized a fluorescently activatable coumarin derivative with a benzisoxazole scaffold to enable monitoring of the Kemp elimination reaction in terms of fluorescence change for the first time. We show that this fluorescent sensor can sensitively and selectively quantitate serum albumin in blood samples. It also works in a dry-chemistry format.

Original languageEnglish
Pages (from-to)3464-3467
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume27
Issue number15
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • Chemical biology
  • Enzymes
  • Fluorescent probes
  • Kemp elimination
  • Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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