Fluorescence imaging of single molecules with a super resolution NSOM

N. Hosaka, T. Saiki

Research output: Contribution to conferencePaper

Abstract

Fluorescence imaging of single molecules was conducted using a super resolution near-field scanning optical microscopy (NSOM). The incident light was reduced using neutral density filters to avoid photobleaching of single molecules during the fluorescence imaging. The results show that the 15 nm spatial resolution is obtained which is smaller than the aperture diameter and is attributed to nonradiative energy transfer from the excited molecules to the coated metal of the probe.

Original languageEnglish
PagesI508-I509
Publication statusPublished - 2001 Dec 1
Externally publishedYes
Event4th Pacific Rim Conference on Lasers and Electro-Optics - Chiba, Japan
Duration: 2001 Jul 152001 Jul 19

Other

Other4th Pacific Rim Conference on Lasers and Electro-Optics
CountryJapan
CityChiba
Period01/7/1501/7/19

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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  • Cite this

    Hosaka, N., & Saiki, T. (2001). Fluorescence imaging of single molecules with a super resolution NSOM. I508-I509. Paper presented at 4th Pacific Rim Conference on Lasers and Electro-Optics, Chiba, Japan.