Abstract
Fluorescence imaging of single molecules was conducted using a super resolution near-field scanning optical microscopy (NSOM). The incident light was reduced using neutral density filters to avoid photobleaching of single molecules during the fluorescence imaging. The results show that the 15 nm spatial resolution is obtained which is smaller than the aperture diameter and is attributed to nonradiative energy transfer from the excited molecules to the coated metal of the probe.
| Original language | English |
|---|---|
| Title of host publication | Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest |
| Volume | 1 |
| Publication status | Published - 2001 |
| Externally published | Yes |
| Event | 4th Pacific Rim Conference on Lasers and Electro-Optics - Chiba, Japan Duration: 2001 Jul 15 → 2001 Jul 19 |
Other
| Other | 4th Pacific Rim Conference on Lasers and Electro-Optics |
|---|---|
| Country | Japan |
| City | Chiba |
| Period | 01/7/15 → 01/7/19 |
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ASJC Scopus subject areas
- Physics and Astronomy(all)
Cite this
Fluorescence imaging of single molecules with a super resolution NSOM. / Hosaka, N.; Saiki, Toshiharu.
Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest. Vol. 1 2001.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Fluorescence imaging of single molecules with a super resolution NSOM
AU - Hosaka, N.
AU - Saiki, Toshiharu
PY - 2001
Y1 - 2001
N2 - Fluorescence imaging of single molecules was conducted using a super resolution near-field scanning optical microscopy (NSOM). The incident light was reduced using neutral density filters to avoid photobleaching of single molecules during the fluorescence imaging. The results show that the 15 nm spatial resolution is obtained which is smaller than the aperture diameter and is attributed to nonradiative energy transfer from the excited molecules to the coated metal of the probe.
AB - Fluorescence imaging of single molecules was conducted using a super resolution near-field scanning optical microscopy (NSOM). The incident light was reduced using neutral density filters to avoid photobleaching of single molecules during the fluorescence imaging. The results show that the 15 nm spatial resolution is obtained which is smaller than the aperture diameter and is attributed to nonradiative energy transfer from the excited molecules to the coated metal of the probe.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035695967&partnerID=8YFLogxK
M3 - Conference contribution
VL - 1
BT - Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest
ER -