Fluorescence imaging of single molecules with a super resolution NSOM

N. Hosaka, Toshiharu Saiki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescence imaging of single molecules was conducted using a super resolution near-field scanning optical microscopy (NSOM). The incident light was reduced using neutral density filters to avoid photobleaching of single molecules during the fluorescence imaging. The results show that the 15 nm spatial resolution is obtained which is smaller than the aperture diameter and is attributed to nonradiative energy transfer from the excited molecules to the coated metal of the probe.

Original languageEnglish
Title of host publicationPacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest
Volume1
Publication statusPublished - 2001
Externally publishedYes
Event4th Pacific Rim Conference on Lasers and Electro-Optics - Chiba, Japan
Duration: 2001 Jul 152001 Jul 19

Other

Other4th Pacific Rim Conference on Lasers and Electro-Optics
CountryJapan
CityChiba
Period01/7/1501/7/19

Fingerprint

near fields
microscopy
fluorescence
scanning
molecules
spatial resolution
apertures
energy transfer
filters
probes
metals

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Hosaka, N., & Saiki, T. (2001). Fluorescence imaging of single molecules with a super resolution NSOM. In Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest (Vol. 1)

Fluorescence imaging of single molecules with a super resolution NSOM. / Hosaka, N.; Saiki, Toshiharu.

Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest. Vol. 1 2001.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hosaka, N & Saiki, T 2001, Fluorescence imaging of single molecules with a super resolution NSOM. in Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest. vol. 1, 4th Pacific Rim Conference on Lasers and Electro-Optics, Chiba, Japan, 01/7/15.
Hosaka N, Saiki T. Fluorescence imaging of single molecules with a super resolution NSOM. In Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest. Vol. 1. 2001
Hosaka, N. ; Saiki, Toshiharu. / Fluorescence imaging of single molecules with a super resolution NSOM. Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest. Vol. 1 2001.
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