Fluorine doping into diamond-like carbon coatings inhibits protein adsorption and platelet activation

Terumitsu Hasebe, Satoshi Yohena, Aki Kamijo, Yuko Okazaki, Atsushi Hotta, Koki Takahashi, Tetsuya Suzuki

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The first major event when a medical device comes in contact with blood is the adsorption of plasma proteins. Protein adsorption on the material surface leads to the activation of the blood coagulation cascade and the inflammatory process, which impair the lifetime of the material. Various efforts have been made to minimize protein adsorption and platelet adhesion. Recently, diamond-like carbon (DLC) has received much attention because of their antithrombogenicity. We recently reported that coating silicon substrates with fluorine-doped diamond-like carbon (F-DLC) drastically suppresses platelet adhesion and activation. Here, we evaluated the protein adsorption on the material surfaces and clarified the relationship between protein adsorption and platelet behaviors, using polycarbonate and DLC- or F-DLC-coated polycarbonate. The adsorption of albumin and fibrinogen were assessed using a colorimetric protein assay, and platelet adhesion and activation were examined using a differential interference contrast microscope. A higher ratio of albumin to fibrinogen adsorption was observed on F-DLC than on DLC and polycarbonate films, indicating that the F-DLC film should prevent thrombus formation. Platelet adhesion and activation on the F-DLC films were more strongly suppressed as the amount of fluorine doping was increased. These results show that the F-DLC coating may be useful for blood-contacting devices.

Original languageEnglish
Pages (from-to)1192-1199
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume83
Issue number4
DOIs
Publication statusPublished - 2007 Dec 15

Fingerprint

Diamond
Fluorine
polycarbonate
Platelets
Diamonds
Carbon
Chemical activation
Doping (additives)
Proteins
Adsorption
Coatings
Adhesion
Polycarbonates
Diamond like carbon films
Blood
Fibrinogen
Albumins
Silicon
Coagulation
Blood Proteins

Keywords

  • Diamond-like carbon
  • Fluorine
  • Platelet activation
  • Protein adsorption

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Fluorine doping into diamond-like carbon coatings inhibits protein adsorption and platelet activation. / Hasebe, Terumitsu; Yohena, Satoshi; Kamijo, Aki; Okazaki, Yuko; Hotta, Atsushi; Takahashi, Koki; Suzuki, Tetsuya.

In: Journal of Biomedical Materials Research - Part A, Vol. 83, No. 4, 15.12.2007, p. 1192-1199.

Research output: Contribution to journalArticle

Hasebe, Terumitsu ; Yohena, Satoshi ; Kamijo, Aki ; Okazaki, Yuko ; Hotta, Atsushi ; Takahashi, Koki ; Suzuki, Tetsuya. / Fluorine doping into diamond-like carbon coatings inhibits protein adsorption and platelet activation. In: Journal of Biomedical Materials Research - Part A. 2007 ; Vol. 83, No. 4. pp. 1192-1199.
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