Fluoro-Jade: New fluorescent marker of Rosenthal fibers

Kenji Tanaka, Nobuhiko Ochi, Toshiyuki Hayashi, Eiji Ikeda, Kazuhiro Ikenaka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rosenthal fibers are homogeneous eosinophilic masses found in astrocytes that are composed of glial fibrillary acidic protein (GFAP) aggregates along with chaperone proteins and other unknown components. Rosenthal fiber formation is a pathological hallmark of Alexander disease and its detection is diagnostically significant. However, the lack of a specific fluorescent marker has greatly limited the histochemical characterization of Rosenthal fibers. Here, we report for the first time a fluorescent marker of Rosenthal fibers called Fluoro Jade. Fluoro Jade-positive masses were seen in samples of Alexander disease brain, pilocytic astrocytoma, and in brain tissue from a mouse model of Alexander disease. Fluoro Jade co-labeled tissue samples stained with GFAP immunofluorescence. Our results indicated that Fluoro Jade labeled Rosenthal fibers, and that Rosenthal fibers could be labeled with antibodies of interest in combination with Fluoro Jade staining.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalNeuroscience Letters
Volume407
Issue number2
DOIs
Publication statusPublished - 2006 Oct 23
Externally publishedYes

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Keywords

  • Aggregates
  • Alexander disease
  • Fluoro-Jade
  • GFAP
  • Rosenthal fibers

ASJC Scopus subject areas

  • Neuroscience(all)

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