Fluorometric determination of glucose utilization in neurons in vitro and in vivo

Yoshiaki Itoh, Takato Abe, Rie Takaoka, Norio Tanahashi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Glucose is the major energy source the adult brain utilizes under physiologic conditions. Recent findings, however, have suggested that neurons obtain most of their energy from the oxidation of extracellular lactate derived from astroglial metabolism of glucose transported into the brain from the blood. In the present studies we have used 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-cleoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, which is often used to trace glucose utilization in neural tissues, to examine glucose metabolism in neurons in vitro and in vivo. Cultured neurons and astroglia were incubated with 2-NBDG for up to 15 minutes, and nonmetabolized 2-NBDG was washed out. We found that fluorescence intensity increased linearly with incubation time in both neurons and astroglia, indicating that both types of brain cells could utilize glucose as their energy source in vitro. To determine if the same were true in vivo, Sprague-Dawley rats were injected intravenously with a pulse bolus of 2-NBDG and decapitated 45 minutes later. Examination of brain sections demonstrated that phosphorylated 2-NBDG accumulated in hippocampal neurons and cerebellar Purkinje cells, indicating that neurons can utilize glucose in vivo as energy source.

Original languageEnglish
Pages (from-to)993-1003
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume24
Issue number9
DOIs
Publication statusPublished - 2004 Sep

Fingerprint

Neurons
Glucose
Brain
Astrocytes
Purkinje Cells
Deoxyglucose
In Vitro Techniques
Sprague Dawley Rats
Lactic Acid
Fluorescence
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose

Keywords

  • 2-Deoxyglucose
  • 2-NBDG
  • Astroglia
  • Fluorescence
  • Glucose utilization
  • Neuron

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Fluorometric determination of glucose utilization in neurons in vitro and in vivo. / Itoh, Yoshiaki; Abe, Takato; Takaoka, Rie; Tanahashi, Norio.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 24, No. 9, 09.2004, p. 993-1003.

Research output: Contribution to journalArticle

Itoh, Yoshiaki ; Abe, Takato ; Takaoka, Rie ; Tanahashi, Norio. / Fluorometric determination of glucose utilization in neurons in vitro and in vivo. In: Journal of Cerebral Blood Flow and Metabolism. 2004 ; Vol. 24, No. 9. pp. 993-1003.
@article{4ce5d2a77e2f4024b599d92f03bcaa5e,
title = "Fluorometric determination of glucose utilization in neurons in vitro and in vivo",
abstract = "Glucose is the major energy source the adult brain utilizes under physiologic conditions. Recent findings, however, have suggested that neurons obtain most of their energy from the oxidation of extracellular lactate derived from astroglial metabolism of glucose transported into the brain from the blood. In the present studies we have used 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-cleoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, which is often used to trace glucose utilization in neural tissues, to examine glucose metabolism in neurons in vitro and in vivo. Cultured neurons and astroglia were incubated with 2-NBDG for up to 15 minutes, and nonmetabolized 2-NBDG was washed out. We found that fluorescence intensity increased linearly with incubation time in both neurons and astroglia, indicating that both types of brain cells could utilize glucose as their energy source in vitro. To determine if the same were true in vivo, Sprague-Dawley rats were injected intravenously with a pulse bolus of 2-NBDG and decapitated 45 minutes later. Examination of brain sections demonstrated that phosphorylated 2-NBDG accumulated in hippocampal neurons and cerebellar Purkinje cells, indicating that neurons can utilize glucose in vivo as energy source.",
keywords = "2-Deoxyglucose, 2-NBDG, Astroglia, Fluorescence, Glucose utilization, Neuron",
author = "Yoshiaki Itoh and Takato Abe and Rie Takaoka and Norio Tanahashi",
year = "2004",
month = "9",
doi = "10.1097/01.WCB.0000127661.07591.DE",
language = "English",
volume = "24",
pages = "993--1003",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Fluorometric determination of glucose utilization in neurons in vitro and in vivo

AU - Itoh, Yoshiaki

AU - Abe, Takato

AU - Takaoka, Rie

AU - Tanahashi, Norio

PY - 2004/9

Y1 - 2004/9

N2 - Glucose is the major energy source the adult brain utilizes under physiologic conditions. Recent findings, however, have suggested that neurons obtain most of their energy from the oxidation of extracellular lactate derived from astroglial metabolism of glucose transported into the brain from the blood. In the present studies we have used 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-cleoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, which is often used to trace glucose utilization in neural tissues, to examine glucose metabolism in neurons in vitro and in vivo. Cultured neurons and astroglia were incubated with 2-NBDG for up to 15 minutes, and nonmetabolized 2-NBDG was washed out. We found that fluorescence intensity increased linearly with incubation time in both neurons and astroglia, indicating that both types of brain cells could utilize glucose as their energy source in vitro. To determine if the same were true in vivo, Sprague-Dawley rats were injected intravenously with a pulse bolus of 2-NBDG and decapitated 45 minutes later. Examination of brain sections demonstrated that phosphorylated 2-NBDG accumulated in hippocampal neurons and cerebellar Purkinje cells, indicating that neurons can utilize glucose in vivo as energy source.

AB - Glucose is the major energy source the adult brain utilizes under physiologic conditions. Recent findings, however, have suggested that neurons obtain most of their energy from the oxidation of extracellular lactate derived from astroglial metabolism of glucose transported into the brain from the blood. In the present studies we have used 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-cleoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, which is often used to trace glucose utilization in neural tissues, to examine glucose metabolism in neurons in vitro and in vivo. Cultured neurons and astroglia were incubated with 2-NBDG for up to 15 minutes, and nonmetabolized 2-NBDG was washed out. We found that fluorescence intensity increased linearly with incubation time in both neurons and astroglia, indicating that both types of brain cells could utilize glucose as their energy source in vitro. To determine if the same were true in vivo, Sprague-Dawley rats were injected intravenously with a pulse bolus of 2-NBDG and decapitated 45 minutes later. Examination of brain sections demonstrated that phosphorylated 2-NBDG accumulated in hippocampal neurons and cerebellar Purkinje cells, indicating that neurons can utilize glucose in vivo as energy source.

KW - 2-Deoxyglucose

KW - 2-NBDG

KW - Astroglia

KW - Fluorescence

KW - Glucose utilization

KW - Neuron

UR - http://www.scopus.com/inward/record.url?scp=4544316033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4544316033&partnerID=8YFLogxK

U2 - 10.1097/01.WCB.0000127661.07591.DE

DO - 10.1097/01.WCB.0000127661.07591.DE

M3 - Article

C2 - 15356420

AN - SCOPUS:4544316033

VL - 24

SP - 993

EP - 1003

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 9

ER -