Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells

Dusko Ilic, Yasuhide Furuta, Toshio Suda, Tadao Atsumi, Jiro Fujimoto, Yoji Ikawa, Tadashi Yamamoto, Shinichi Aizawa

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Focal adhesion kinase, FAK, is a unique protein tyrosine kinase found in cellular focal adhesions. It is widely expressed and highly phosphorylated during embryogenesis. To examine the function of FAK in cell differentiation, we made FAK-deficient embryonic stem (ES) cells by homologous recombination. However, FAK deficiency did not interfere with differentiation of the ES cells into cells of three germ layers when implanted subcutaneously into nude mice or when treated with retinoic acid in vitro, nor was there any evidence of defects in hematopoiesis in vitro.

Original languageEnglish
Pages (from-to)300-309
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume209
Issue number1
DOIs
Publication statusPublished - 1995 Apr 6

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Ilic, D., Furuta, Y., Suda, T., Atsumi, T., Fujimoto, J., Ikawa, Y., Yamamoto, T., & Aizawa, S. (1995). Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells. Biochemical and Biophysical Research Communications, 209(1), 300-309. https://doi.org/10.1006/bbrc.1995.1503