Focusing the diversity of Gardnerella vaginalis through the lens of ecotypes

Omar E. Cornejo, Roxana J. Hickey, Haruo Suzuki, Larry J. Forney

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Gardnerella vaginalis has long been associated with bacterial vaginosis, a condition that increases the risk of women to preterm birth, sexually transmitted infections, and other adverse sequelae. However, G. vaginalis is also commonly found in healthy asymptomatic women of all ages. This raises the question if genetic differences among strains might distinguish potentially pathogenic from commensal strains. To disentangle the diversity of G. vaginalis, we invoked the concept of ecotypes—lineages of genetically and ecologically distinct strains within a named species—to better understand their evolutionary history and identify functional characteristics. We compared the genomes of G. vaginalis to six species in the closely related Bifidobacterium genus and found that G. vaginalis has a large accessory genome relative to Bifidobacterium, including many unique genes possibly involved in metabolism, drug resistance, and virulence. We then performed a comparative genomic analysis of 35 strains of G. vaginalis to infer a phylogeny based on the combined analysis of the core genome, using nucleotide substitution models, and the accessory genome, using gene gain/loss models. With the inferred tree topology, we performed comparisons of functional gene content among lineages that diverged at varying depths in the phylogeny and found significant differences in the representation of genes putatively involved in pathogenicity. Our functional enrichment analysis suggests that some lineages of G. vaginalis may possess enhanced pathogenic capabilities, including genes involved in mucus degradation like sialidases, while others may be commensal strains, lacking many of these pathogenic capabilities. The combined phylogenetic evidence and functional enrichment analysis allowed us to identify distinct ecotypes that have evolved in G. vaginalis as the result of the differential gene gain/loss for specific functions, including the capability to cause disease. We finally discuss how this analysis framework could be used to gain insight into the etiology of bacterial vaginosis and improve diagnosis.

Original languageEnglish
Pages (from-to)312-324
Number of pages13
JournalEvolutionary Applications
Volume11
Issue number3
DOIs
Publication statusPublished - 2018 Mar 1

Fingerprint

Gardnerella vaginalis
Ecotype
ecotype
Lens
ecotypes
Lenses
gene
genome
Genome
Genes
Bacterial Vaginosis
commensal
Bifidobacterium
genes
Phylogeny
Virulence
phylogeny
sexually transmitted diseases
drug resistance
premature birth

Keywords

  • bacterial vaginosis
  • Gardnerella vaginalis
  • genetic diversity
  • genomes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Agricultural and Biological Sciences(all)

Cite this

Focusing the diversity of Gardnerella vaginalis through the lens of ecotypes. / Cornejo, Omar E.; Hickey, Roxana J.; Suzuki, Haruo; Forney, Larry J.

In: Evolutionary Applications, Vol. 11, No. 3, 01.03.2018, p. 312-324.

Research output: Contribution to journalArticle

Cornejo, Omar E. ; Hickey, Roxana J. ; Suzuki, Haruo ; Forney, Larry J. / Focusing the diversity of Gardnerella vaginalis through the lens of ecotypes. In: Evolutionary Applications. 2018 ; Vol. 11, No. 3. pp. 312-324.
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