TY - JOUR
T1 - Folate, vitamin B6, vitamin B12, and vitamin B 2 intake, genetic polymorphisms of related enzymes, and risk of colorectal cancer in a hospital-based case-control study in Japan
AU - Otani, Tetsuya
AU - Iwasaki, Motoki
AU - Hanaoka, Tomoyuki
AU - Kobayashi, Minatsu
AU - Ishihara, Junko
AU - Natsukawa, Syusuke
AU - Shaura, Kozo
AU - Koizumi, Yoichi
AU - Kasuga, Yoshio
AU - Yoshimura, Kimio
AU - Yoshida, Teruhiko
AU - Tsugane, Shoichiro
N1 - Funding Information:
We gratefully acknowledge the generous assistance of the staff members of each hospital and the Agricultural Technology Institute of Nagano Farmers’ Federation, including the survey staffs, Ms. Aoki, Mr. Ueki, Ms. Kimijima, Ms. Komatsu, Mr. Shimazaki, Ms. Horano, Mr. Yajima, Dr. Ikegawa, and Dr. Matsuzawa. They also thank Ms. Hashimoto and Ms. Kanai for technical assistance with laboratory assays. This study was supported by the Agricultural Technology Institute of the Nagano Farmers’Federation, by a Grant-in-Aid for Cancer Research and for the Third-Term Comprehensive 10-yr Strategy for Cancer Control from the Ministry of Health, Labor and Welfare of Japan, by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by the program for promotion of Fundamental Studies in Health Sciences of the Pharmaceuticals and Medical Devices Agency (PMDA). Address correspondence to Tetsuya Otani, Epidemiology and Prevention Division, Research Center for Cancer Prevention and Screening, National Cancer Center, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. Phone: +81-3-3542-2511 Ext 3378. FAX: +81-3-3547-8578. E-mail: teotani@gan2.res.ncc.go.jp.
PY - 2005
Y1 - 2005
N2 - We conducted a case-control study to investigate the association of nutrient intake involved in the one-carbon pathway of folate for DNA methylation and DNA synthesis and the related enzyme genetic polymorphisms with colorectal cancer. Cases were 107 patients newly diagnosed with colorectal cancer. Controls were 224 subjects matched with cases by sex, age, and residential area. Nutrient intake was assessed by a self-administered, semiquantitative food-frequency questionnaire. Four genetic polymorphisms-MTHFR C677T and A1298C, MTRR A66G, and ALDH2 Glu487Lys-were determined using blood samples. Odds ratios were calculated using conditional logistic regression analysis adjusted for smoking, alcohol consumption, body mass index, and dietary fiber intake. Although folate intake was inversely associated with colorectal cancer, this association was attenuated after further controlling for dietary fiber intake. Neither vitamin B6, vitamin B12, nor vitamin B 2, nor any genetic polymorphism was significantly associated with colorectal cancer. MTRR polymorphism interacted with the association of folate (P for interaction = 0.04) or vitamin B6 (P for interaction = 0.02) with colorectal cancer, although the other polymorphisms did not interact with any nutrient intake. In conclusion, the study did not support the existing hypothesis of gene-nutrient interaction in colorectal carcinogenesis.
AB - We conducted a case-control study to investigate the association of nutrient intake involved in the one-carbon pathway of folate for DNA methylation and DNA synthesis and the related enzyme genetic polymorphisms with colorectal cancer. Cases were 107 patients newly diagnosed with colorectal cancer. Controls were 224 subjects matched with cases by sex, age, and residential area. Nutrient intake was assessed by a self-administered, semiquantitative food-frequency questionnaire. Four genetic polymorphisms-MTHFR C677T and A1298C, MTRR A66G, and ALDH2 Glu487Lys-were determined using blood samples. Odds ratios were calculated using conditional logistic regression analysis adjusted for smoking, alcohol consumption, body mass index, and dietary fiber intake. Although folate intake was inversely associated with colorectal cancer, this association was attenuated after further controlling for dietary fiber intake. Neither vitamin B6, vitamin B12, nor vitamin B 2, nor any genetic polymorphism was significantly associated with colorectal cancer. MTRR polymorphism interacted with the association of folate (P for interaction = 0.04) or vitamin B6 (P for interaction = 0.02) with colorectal cancer, although the other polymorphisms did not interact with any nutrient intake. In conclusion, the study did not support the existing hypothesis of gene-nutrient interaction in colorectal carcinogenesis.
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U2 - 10.1207/s15327914nc5301_5
DO - 10.1207/s15327914nc5301_5
M3 - Article
C2 - 16351505
AN - SCOPUS:30344440353
SN - 0163-5581
VL - 53
SP - 42
EP - 50
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 1
ER -
