Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone α subunit mRNA levels in artificially maturing Japanese eel Anguilla japonica and naturally maturing New Zealand longfinned eel Anguilla dieffenbachii

Koji Saito, P. Mark Lokman, Graham Young, Yuichi Ozaki, Hajime Matsubara, Hiromi Okumura, Yukinori Kazeto, Yasutoshi Yoshiura, Katsumi Aida, Shinji Adachi, Kohei Yamauchi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

cDNAs for three subunits of gonadotropin, namely follicle-stimulating hormone (FSH) β, luteinizing hormone (LH) β and glycoprotein hormone (GP) α, of the New Zealand longfinned eel Anguilla dieffenbachii were cloned. Based on their nucleotide sequences, which were more than 98% identical to those of the Japanese eel Anguilla japonica, a real-time reverse transcription-polymerase chain reaction method for the quantitative assay of gonadotropin subunit mRNAs of the two eel species was developed using the same primer sets. Accordingly, changes in pituitary gonadotropin mRNA levels were analyzed in female Japanese eels induced to develop artificially by salmon pituitary homogenate (SPH) treatment and in naturally maturing female New Zealand longfinned eels collected from the wild. In Japanese eels, the FSH β mRNA level was very high at the previtellogenic stage (PV) and decreased remarkably after SPH injection, whereas the reverse was seen in New Zealand longfinned eels. The mRNA levels of LH β and GP α increased during gonadal development in both species, albeit to a much greater extent in Japanese eels. As the Japanese and New Zealand longfinned eels are closely related species of the same genus, the different gonadotropin expression patterns are likely to reflect differences in maturational conditions rather than species. Our findings are indicative of aberrant gonadal development and may explain the poor quality eggs obtained from artificially matured Japanese eels.

Original languageEnglish
Pages (from-to)146-153
Number of pages8
JournalFisheries Science
Volume69
Issue number1
DOIs
Publication statusPublished - 2003 Feb
Externally publishedYes

Fingerprint

Anguilla japonica
follicle-stimulating hormone
luteinizing hormone
eel
hormone
glycoproteins
hormones
gonadotropins
salmon
pituitary gonadotropins
egg quality
Anguilla dieffenbachii
quantitative analysis
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
injection
nucleotide sequences
polymerase chain reaction
assays
assay

Keywords

  • Gene expression
  • Gonadal maturation
  • Gonadotropin
  • Japanese eel
  • New Zealand longfinned eel
  • Real-time PCR

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone α subunit mRNA levels in artificially maturing Japanese eel Anguilla japonica and naturally maturing New Zealand longfinned eel Anguilla dieffenbachii. / Saito, Koji; Lokman, P. Mark; Young, Graham; Ozaki, Yuichi; Matsubara, Hajime; Okumura, Hiromi; Kazeto, Yukinori; Yoshiura, Yasutoshi; Aida, Katsumi; Adachi, Shinji; Yamauchi, Kohei.

In: Fisheries Science, Vol. 69, No. 1, 02.2003, p. 146-153.

Research output: Contribution to journalArticle

Saito, Koji ; Lokman, P. Mark ; Young, Graham ; Ozaki, Yuichi ; Matsubara, Hajime ; Okumura, Hiromi ; Kazeto, Yukinori ; Yoshiura, Yasutoshi ; Aida, Katsumi ; Adachi, Shinji ; Yamauchi, Kohei. / Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone α subunit mRNA levels in artificially maturing Japanese eel Anguilla japonica and naturally maturing New Zealand longfinned eel Anguilla dieffenbachii. In: Fisheries Science. 2003 ; Vol. 69, No. 1. pp. 146-153.
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AU - Saito, Koji

AU - Lokman, P. Mark

AU - Young, Graham

AU - Ozaki, Yuichi

AU - Matsubara, Hajime

AU - Okumura, Hiromi

AU - Kazeto, Yukinori

AU - Yoshiura, Yasutoshi

AU - Aida, Katsumi

AU - Adachi, Shinji

AU - Yamauchi, Kohei

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AB - cDNAs for three subunits of gonadotropin, namely follicle-stimulating hormone (FSH) β, luteinizing hormone (LH) β and glycoprotein hormone (GP) α, of the New Zealand longfinned eel Anguilla dieffenbachii were cloned. Based on their nucleotide sequences, which were more than 98% identical to those of the Japanese eel Anguilla japonica, a real-time reverse transcription-polymerase chain reaction method for the quantitative assay of gonadotropin subunit mRNAs of the two eel species was developed using the same primer sets. Accordingly, changes in pituitary gonadotropin mRNA levels were analyzed in female Japanese eels induced to develop artificially by salmon pituitary homogenate (SPH) treatment and in naturally maturing female New Zealand longfinned eels collected from the wild. In Japanese eels, the FSH β mRNA level was very high at the previtellogenic stage (PV) and decreased remarkably after SPH injection, whereas the reverse was seen in New Zealand longfinned eels. The mRNA levels of LH β and GP α increased during gonadal development in both species, albeit to a much greater extent in Japanese eels. As the Japanese and New Zealand longfinned eels are closely related species of the same genus, the different gonadotropin expression patterns are likely to reflect differences in maturational conditions rather than species. Our findings are indicative of aberrant gonadal development and may explain the poor quality eggs obtained from artificially matured Japanese eels.

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