Follicle-stimulating hormone enhances recovery from low-dose doxorubicin-induced spermatogenic disorders in mice

Jun Hagiuda, Hiromichi Ishikawa, Satoru Kaneko, Masako Okazaki, Mototsugu Oya, Ken Nakagawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose We aimed to investigate the effects of FSH for promoting spermatogenesis in mice with low-dose doxorubicininduced spermatogenesis impairment. Methods Eight-wk-old male imprinting control region mice were divided into three groups. Groups D and F received 0.5 mg/kg of doxorubicin twice weekly for 5 weeks. Group C received saline instead of doxorubicin. After inducing spermatogenesis impairment, group D was treated daily with saline for 4 weeks. Group F was given 1 IU of recombinant human FSH daily for 4 weeks. Spermatogenesis recovery was evaluated based on the testis weight, sperm count, histological assessment, and mating. The percentage of sperm with unfragmented deoxyribonucleic acid (DNA) was analyzed by single-cell pulsed-field gel electrophoresis, and the serum FSH levels were measured. Results The elevation of serum FSH advanced slowly. The testis weight, sperm count, percentage of seminiferous tubules with spermatogenesis, percentage of sperm with unfragmented DNA and pregnancy rate were significantly increased by the administration of FSH. Conclusion Our study findings indicated that the immediate administration of exogenous FSH can promote the recovery from impaired spermatogenesis induced by low-dose doxorubicin before endogenous FSH increases to the maximum level.

Original languageEnglish
Article numberA004
Pages (from-to)917-923
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Volume32
Issue number6
DOIs
Publication statusPublished - 2015 Apr 16

Fingerprint

Follicle Stimulating Hormone
Spermatogenesis
Doxorubicin
Sperm Count
Spermatozoa
Testis
Human Follicle Stimulating Hormone
Weights and Measures
Seminiferous Tubules
Pulsed Field Gel Electrophoresis
DNA
Pregnancy Rate
Serum

Keywords

  • Chemotherapy
  • Doxorubicin
  • FSH
  • Spermatogenesis impairment

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Developmental Biology
  • Genetics
  • Genetics(clinical)

Cite this

Follicle-stimulating hormone enhances recovery from low-dose doxorubicin-induced spermatogenic disorders in mice. / Hagiuda, Jun; Ishikawa, Hiromichi; Kaneko, Satoru; Okazaki, Masako; Oya, Mototsugu; Nakagawa, Ken.

In: Journal of Assisted Reproduction and Genetics, Vol. 32, No. 6, A004, 16.04.2015, p. 917-923.

Research output: Contribution to journalArticle

Hagiuda, Jun ; Ishikawa, Hiromichi ; Kaneko, Satoru ; Okazaki, Masako ; Oya, Mototsugu ; Nakagawa, Ken. / Follicle-stimulating hormone enhances recovery from low-dose doxorubicin-induced spermatogenic disorders in mice. In: Journal of Assisted Reproduction and Genetics. 2015 ; Vol. 32, No. 6. pp. 917-923.
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