Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation

Masaya Baba, Mitsuhiro Endoh, Wenjuan Ma, Hirofumi Toyama, Akiyoshi Hirayama, Keizo Nishikawa, Keiyo Takubo, Hiroyuki Hano, Hisashi Hasumi, Terumasa Umemoto, Michihiro Hashimoto, Nobuko Irie, Chiharu Esumi, Miho Kataoka, Naomi Nakagata, Tomoyoshi Soga, Masahiro Yao, Tomomi Kamba, Takashi Minami, Masaru Ishii & 1 others Toshio Suda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Osteoclast differentiation is a dynamic differentiation process, which is accompanied by dramatic changes in metabolic status as well as in gene expression. Recent findings have revealed an essential connection between metabolic reprogramming and dynamic gene expression changes during osteoclast differentiation. However, the upstream regulatory mechanisms that drive these metabolic changes in osteoclastogenesis remain to be elucidated. Here, we demonstrate that induced deletion of a tumor suppressor gene, Folliculin (Flcn), in mouse osteoclast precursors causes severe osteoporosis in 3 weeks through excess osteoclastogenesis. Flcn-deficient osteoclast precursors reveal cell autonomous accelerated osteoclastogenesis with increased sensitivity to receptor activator of NF-κB ligand (RANKL). We demonstrate that Flcn regulates oxidative phosphorylation and purine metabolism through suppression of nuclear localization of the transcription factor Tfe3, thereby inhibiting expression of its target gene Pgc1. Metabolome studies revealed that Flcn-deficient osteoclast precursors exhibit significant augmentation of oxidative phosphorylation and nucleotide production, resulting in an enhanced purinergic signaling loop that is composed of controlled ATP release and autocrine/paracrine purinergic receptor stimulation. Inhibition of this purinergic signaling loop efficiently blocks accelerated osteoclastogenesis in Flcn-deficient osteoclast precursors. Here, we demonstrate an essential and novel role of the Flcn-Tfe3-Pgc1 axis in osteoclastogenesis through the metabolic reprogramming of oxidative phosphorylation and purine metabolism.

Original languageEnglish
Pages (from-to)1785-1798
Number of pages14
JournalJournal of Bone and Mineral Research
Volume33
Issue number10
DOIs
Publication statusPublished - 2018 Oct 1

Fingerprint

Estrone
Osteoclasts
Osteogenesis
Oxidative Phosphorylation
Gene Expression
Purinergic Receptors
Metabolome
Tumor Suppressor Genes
Osteoporosis
Transcription Factors
Nucleotides
Adenosine Triphosphate
Ligands
Genes

Keywords

  • FOLLICULIN (FLCN)
  • METABOLISM
  • OSTEOCLAST
  • OSTEOPOROSIS
  • TRANSCRIPTION FACTORS

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

Baba, M., Endoh, M., Ma, W., Toyama, H., Hirayama, A., Nishikawa, K., ... Suda, T. (2018). Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation. Journal of Bone and Mineral Research, 33(10), 1785-1798. https://doi.org/10.1002/jbmr.3477

Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation. / Baba, Masaya; Endoh, Mitsuhiro; Ma, Wenjuan; Toyama, Hirofumi; Hirayama, Akiyoshi; Nishikawa, Keizo; Takubo, Keiyo; Hano, Hiroyuki; Hasumi, Hisashi; Umemoto, Terumasa; Hashimoto, Michihiro; Irie, Nobuko; Esumi, Chiharu; Kataoka, Miho; Nakagata, Naomi; Soga, Tomoyoshi; Yao, Masahiro; Kamba, Tomomi; Minami, Takashi; Ishii, Masaru; Suda, Toshio.

In: Journal of Bone and Mineral Research, Vol. 33, No. 10, 01.10.2018, p. 1785-1798.

Research output: Contribution to journalArticle

Baba, M, Endoh, M, Ma, W, Toyama, H, Hirayama, A, Nishikawa, K, Takubo, K, Hano, H, Hasumi, H, Umemoto, T, Hashimoto, M, Irie, N, Esumi, C, Kataoka, M, Nakagata, N, Soga, T, Yao, M, Kamba, T, Minami, T, Ishii, M & Suda, T 2018, 'Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation', Journal of Bone and Mineral Research, vol. 33, no. 10, pp. 1785-1798. https://doi.org/10.1002/jbmr.3477
Baba M, Endoh M, Ma W, Toyama H, Hirayama A, Nishikawa K et al. Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation. Journal of Bone and Mineral Research. 2018 Oct 1;33(10):1785-1798. https://doi.org/10.1002/jbmr.3477
Baba, Masaya ; Endoh, Mitsuhiro ; Ma, Wenjuan ; Toyama, Hirofumi ; Hirayama, Akiyoshi ; Nishikawa, Keizo ; Takubo, Keiyo ; Hano, Hiroyuki ; Hasumi, Hisashi ; Umemoto, Terumasa ; Hashimoto, Michihiro ; Irie, Nobuko ; Esumi, Chiharu ; Kataoka, Miho ; Nakagata, Naomi ; Soga, Tomoyoshi ; Yao, Masahiro ; Kamba, Tomomi ; Minami, Takashi ; Ishii, Masaru ; Suda, Toshio. / Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation. In: Journal of Bone and Mineral Research. 2018 ; Vol. 33, No. 10. pp. 1785-1798.
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