Formation and recovery of a cell sheet by a particle monolayer with the surface roughness

Manabu Miura, Keiji Fujimoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We studied the topographical effect of roughness displayed by a closely packed particle monolayer on formation of a cell monolayer (cell sheet). Particle monolayers were prepared by Langmuir-Blodgett deposition using particles, which were 527 nm (SA053) and 1270 nm (SA127) in diameter. Human umbilical vein endothelial cells (HUVECs) were seeded at a high density (2.0 × 105 cells/cm2) onto particle monolayers. It was found that cells gradually became into contact with adjacent cells on the SA053 monolayer and the formed cell sheet could be readily detached from the particle monolayer by gentle pipetting. On the other hand, cells adhering onto the tissue culture polystyrene (TCPS) and the SA127 particle monolayer were difficult to peel off. At a low cell seeding density (5.0 × 104 cells/cm2), pre-coating with bovine plasma fibronectin (FN) allowed cell growth on an SA053 particle monolayer, and a confluent monolayer was able to be peeled as a cell sheet from the particle monolayer just by pipetting. By immunostaining of human fibronectin, we found that fibronectin was secreted and concentrated onto the substrate side of a cell sheet. The obtained cell sheet adhered and grew on the TCPS again within 20 min.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume66
Issue number1
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

Monolayers
surface roughness
Surface roughness
recovery
Recovery
cells
Fibronectins
Tissue culture
Polystyrenes
polystyrene
Endothelial cells
Cell growth
Human Umbilical Vein Endothelial Cells
inoculation
veins
Cells
roughness
Plasmas
Cell Count
Coatings

Keywords

  • Cell adhesion
  • Cell culture
  • Cell sheet
  • Microsphere
  • Particle
  • Particle monolayer
  • Surface roughness
  • Surface topography

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Formation and recovery of a cell sheet by a particle monolayer with the surface roughness. / Miura, Manabu; Fujimoto, Keiji.

In: Colloids and Surfaces B: Biointerfaces, Vol. 66, No. 1, 01.10.2008, p. 125-133.

Research output: Contribution to journalArticle

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