Abstract
Cellular aggregates that mimic cell–cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology.
| Original language | English |
|---|---|
| Journal | Ultrasound in Medicine and Biology |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
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Keywords
- Mouse myoblasts
- Scaffold-free aggregates
- Three-dimensional aggregates
- Tissue engineering
- Ultrasound standing wave trapping
- Ultrasound transducer
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Biophysics
- Acoustics and Ultrasonics
Cite this
Formation of Large Scaffold-Free 3-D Aggregates in a Cell Culture Dish by Ultrasound Standing Wave Trapping. / Nakao, Misa; Imashiro, Chikahiro; Kuribara, Taiki; Kurashina, Yuta; Totani, Kiichiro; Takemura, Kenjiro.
In: Ultrasound in Medicine and Biology, 01.01.2019.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Formation of Large Scaffold-Free 3-D Aggregates in a Cell Culture Dish by Ultrasound Standing Wave Trapping
AU - Nakao, Misa
AU - Imashiro, Chikahiro
AU - Kuribara, Taiki
AU - Kurashina, Yuta
AU - Totani, Kiichiro
AU - Takemura, Kenjiro
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Cellular aggregates that mimic cell–cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology.
AB - Cellular aggregates that mimic cell–cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology.
KW - Mouse myoblasts
KW - Scaffold-free aggregates
KW - Three-dimensional aggregates
KW - Tissue engineering
KW - Ultrasound standing wave trapping
KW - Ultrasound transducer
UR - http://www.scopus.com/inward/record.url?scp=85061797661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061797661&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2019.01.013
DO - 10.1016/j.ultrasmedbio.2019.01.013
M3 - Article
C2 - 30799124
AN - SCOPUS:85061797661
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
SN - 0301-5629
ER -