Formation of superoxide anion in the hepatic sinusoid after lipopolysaccharide challenge

Hirokazu Yokoyama, Takeshi Mizukami, Yoshitaka Kamegaya, Masahiko Fukuda, Yukishige Okamura, Michinaga Matsumoto, Shinzo Kato, Hiromasa Ishii

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using the cytochrome c method, superoxide anion that is released into the hepatic sinusoid was measured after a lipopolysaccharide challenge in a liver perfusion system. Moreover, damages of epithelial cells of the hepatic sinusoid were estimated with scanning electron microscopic analysis and levels of purine nucleoside phosphorylase/GPT ratio. Lipopolysaccharide administration increased the conversion of oxidized cytochrome c into reduced cytochrome c in the perfusate, indicating that superoxide anion was formed in the hepatic sinusoid. This change was associated with increase in levels of portal tumor necrosis factor-α and attenuated by the simultaneous administration of Superoxide dismutase. Scanning electron microscope analysis revealed that diameters of sinusoidal fenestrae increased in rats treated with lipopolysaccharide, compared with controls. Moreover, levels of purine nucleoside phosphorylase/GPT ratio was significantly increased in the liver perfusate in lipopolysac-charide-treated rats, compared with controls. Superoxide anion in hepatic sinusoid may be one of the pathogenic factors behind damages of epithelial cells of the hepatic sinusoid caused by lipopolysaccharide.

Original languageEnglish
Pages (from-to)133S-136S
JournalAlcoholism: Clinical and Experimental Research
Volume22
Issue number3 SUPPL. 1
DOIs
Publication statusPublished - 1998

Keywords

  • Cytochrome c
  • Hepatic Sinusoidal Epithelial Cells
  • Lipopolysaccharide
  • Purine nucleoside Phosphorylase
  • Superoxide Anion

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

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