Forming of 3D neuronal pathway by neuronal block assembly

M. Kato-Negishi, H. Onoe, Y. Morimoto, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes a method of three-dimensional (3D) neuronal pathway formation in vitro by bottom-up neuronal tissue block assembly (Fig.1). We first formed the neuronal tissue blocks by molding uniform-sized neurospheroids into the PDMS mold chambers. We then assembled a millimeter-sized cortex-hippocampal tissue by connecting cortical and hippo-campal tissue blocks. We found that cortical and hippocampal neurons extended their axons to each other, and that 3D neuronal pathway was successfully formed in the 3D environment. We believe that our neuronal block assembly enables the fabrication of in vivo-like neuronal pathway, and that this system is useful tool for neuronal tissue engineering, pharmacological assay for the screening of drugs and toxins to neuronal pathway.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1496-1498
Number of pages3
Publication statusPublished - 2011 Dec 1
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

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Keywords

  • 3D tissue engineering
  • Ca imaging
  • Cortex
  • Hippocampus
  • Neuron
  • Neuronal pathway

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Kato-Negishi, M., Onoe, H., Morimoto, Y., & Takeuchi, S. (2011). Forming of 3D neuronal pathway by neuronal block assembly. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1496-1498). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).