Calcium dependent proteases (calpains. CAPNs, E.C.220.127.116.11) constitute a family of proteins which share a homologous cysteine-protease domain (large subunits. LI. L2. and L3) and an E-F hand Ca2+-binding domain (L1. L2. L3. and small subunit. S). We have mapped the genes for four calpain proteins (LI, L2, L3, and S) on four distinct human chromosomes by a combination of spot-blot hybridization to flow-sorted chromosomes and Southern hybridization of DNAs from a human × mouse hybrid cell panel. The genes for calpain LI (CAPN1. large subunit of calpain I). L2 (CAPN2, large subunit of calpain II), L3 (CAPN3. a protein related to the large subunits), and S (CAPN4. a small subunit common to calpains I and II) were assigned to human chromosomes 11, 1.15. and 19. respectively.
ASJC Scopus subject areas
- Molecular Biology