Four synonymous genes encode calmodulin in the teleost fish, medaka (Oryzias latipes): conservation of the multigene one-protein principle

Koichi Matsuo, Kozo Sato, Hiroko Ikeshima, Kouji Shimoda, Toshiya Takano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We cloned four distinct calmodulin (CaM)-encoding cDNAs from a small teleost fish, medaka (Oryzias latipes). The deduced amino acid (aa) sequences were exactly the same in these four genes and identical to the aa sequence of mammalian CaM, because of synonymous codon usages. The four cDNAs from medaka, termed CaM-A, -B, -C and -D, corresponded to mRNAs of 1.8, 1.4, 2.5 and 1.8 kb, respectively, in Northern blot analysis. Our results demonstrated that the 'multigene one-protein' principle of CaM synthesis is applicable to medaka, as well as to mammals whose CaM is encoded by at least three different genes.

Original languageEnglish
Pages (from-to)279-281
Number of pages3
JournalGene
Volume119
Issue number2
DOIs
Publication statusPublished - 1992 Oct 1

Fingerprint

Oryzias
Calmodulin
Fishes
Genes
Proteins
Amino Acid Sequence
Complementary DNA
Codon
Northern Blotting
Mammals
Messenger RNA

Keywords

  • coding sequences
  • gene evolution
  • polymerase chain reaction
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Four synonymous genes encode calmodulin in the teleost fish, medaka (Oryzias latipes) : conservation of the multigene one-protein principle. / Matsuo, Koichi; Sato, Kozo; Ikeshima, Hiroko; Shimoda, Kouji; Takano, Toshiya.

In: Gene, Vol. 119, No. 2, 01.10.1992, p. 279-281.

Research output: Contribution to journalArticle

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