Free radicals in phacoemulsification and aspiration procedures

Hiroshi Takahashi, Atsuhiro Sakamoto, Ryoki Takahashi, Takeo Ohmura, Shigeto Shimmura, Kunitoshi Ohara

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objectives: To detect free radicals in phacoemulsification and aspiration procedures using electron-spin resonance and to observe the effect of ophthalmic viscosurgical devices (viscoelastic agents) on free radical intensity. Methods: (1) A test tube containing BSS Plus (Alcon Laboratories, Inc, Fort Worth, Tex) with 1% of the spin-trapping agent, 5,5′-d/methyl-1-pyrroline N-oxide, without irrigation and aspiration, was exposed to ultrasound (100% for 20 seconds). A preparation of hyaluronate sodium (Healon [a cohesive agent that contains 1% hyaluronate sodium] {Pharmacia, Uppsala, Sweden} or Viscoat [a dispersive agent that contains 3% hyaluronate sodium and 4% chondroitin sulfate] {Alcon Laboratories, Inc}) was added to the solution to observe inhibitory effects. (2) To simulate a clinical procedure, an eye model with irrigation and aspiration of a combination of 1% 5,5′-dimethyl-1-pyrroline N-oxide and BSS Plus, 25 mL/min, as the irrigating solution was exposed to ultrasound (for 10, 20, or 30 seconds). Healon or Viscoat was injected into the anterior chamber. Free radicals were measured by an electron-spin resonance spectrometer. Results: (1) A characteristic signal corresponding to hydroxyl radicals was detected. Similar inhibition by Healon and Viscoat was observed. (2) Two ophthalmic viscosurgical devices similarly suppressed the signal at 10 seconds. The inhibition by Healon ceased at 20 seconds, whereas Viscoat suppressed the signal throughout the time course. Conclusions: Phacoemulsification produces hydroxyl radicals in the anterior chamber even with irrigation and aspiration. The effect of ophthalmic viscosurgical devices on free radicals depends on the retention of the materials within the anterior chamber. Clinical Relevance: There are complications associated with phacoemulsification.

Original languageEnglish
Pages (from-to)1348-1352
Number of pages5
JournalArchives of Ophthalmology
Volume120
Issue number10
Publication statusPublished - 2002 Oct 1
Externally publishedYes

Fingerprint

Phacoemulsification
Hyaluronic Acid
Free Radicals
Anterior Chamber
Electron Spin Resonance Spectroscopy
Equipment and Supplies
Hydroxyl Radical
Spin Trapping
Chondroitin Sulfates
Sweden
Oxides
sodium hyaluronate drug combination chondroitin sulfate

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Takahashi, H., Sakamoto, A., Takahashi, R., Ohmura, T., Shimmura, S., & Ohara, K. (2002). Free radicals in phacoemulsification and aspiration procedures. Archives of Ophthalmology, 120(10), 1348-1352.

Free radicals in phacoemulsification and aspiration procedures. / Takahashi, Hiroshi; Sakamoto, Atsuhiro; Takahashi, Ryoki; Ohmura, Takeo; Shimmura, Shigeto; Ohara, Kunitoshi.

In: Archives of Ophthalmology, Vol. 120, No. 10, 01.10.2002, p. 1348-1352.

Research output: Contribution to journalArticle

Takahashi, H, Sakamoto, A, Takahashi, R, Ohmura, T, Shimmura, S & Ohara, K 2002, 'Free radicals in phacoemulsification and aspiration procedures', Archives of Ophthalmology, vol. 120, no. 10, pp. 1348-1352.
Takahashi H, Sakamoto A, Takahashi R, Ohmura T, Shimmura S, Ohara K. Free radicals in phacoemulsification and aspiration procedures. Archives of Ophthalmology. 2002 Oct 1;120(10):1348-1352.
Takahashi, Hiroshi ; Sakamoto, Atsuhiro ; Takahashi, Ryoki ; Ohmura, Takeo ; Shimmura, Shigeto ; Ohara, Kunitoshi. / Free radicals in phacoemulsification and aspiration procedures. In: Archives of Ophthalmology. 2002 ; Vol. 120, No. 10. pp. 1348-1352.
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