Freeze-Thawing Procedures Have No Influence on the Phenotypic and Functional Development of Dendritic Cells Generated from Peripheral Blood CD14+ Monocytes

Shin Ichi Hori, Yuji Heike, Masao Takei, Midori Maruyama, Yoshiko Inoue, Je Jung Lee, Hyeoung Joon Kim, Yukie Harada, Hiroyuki Kawai, Akihiro Shimosaka, Masahiro Kami, Ryuji Tanosaki, Hiro Wakasugi, Shigeru Saito, Yoichi Takaue, Tadao Kakizoe

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26 Citations (Scopus)

Abstract

Little is known about the potential influence of cryopreservation on the biologic activities of dendritic cells (DCs). In this study, we examined the effects of freeze-thawing on the phenotypic and functional development of human DCs obtained from granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood CD14+ cells. CD14+ cells were cultured, immediately or after freeze-thawing, with granulocyte-macrophage CSF and interleukin-4 for 9 days, and then with added tumor necrosis factor-α for another 3 days. For both fresh and freeze-thawed monocytes, immature DCs harvested on day 6 and mature DCs harvested on day 9 of culture were examined under the same conditions. Cells were compared with regard to their 1) capacities for antigen endocytosis and chemotactic migration (immature DCs), and 2) allogeneic mixed lymphocyte reaction and antigen-specific cytotoxic T lymphocyte responses (mature DCs). Freeze-thawing did not affect the viability or subsequent maturation of DCs at any stage of development. Furthermore, essentially no difference was observed in phenotype or function between cells generated from fresh or cryopreserved/thawed cells. Although this study design was limited with the use of fetal bovine serum, the observation still suggests that freeze-thawing does not affect viability, phenotype, subsequent maturation, or functions of DCs at any stage of maturation.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalJournal of Immunotherapy
Volume27
Issue number1
DOIs
Publication statusPublished - 2004 Jan
Externally publishedYes

Keywords

  • Cryopreservation
  • Dendritic cells

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Cancer Research

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    Hori, S. I., Heike, Y., Takei, M., Maruyama, M., Inoue, Y., Lee, J. J., Kim, H. J., Harada, Y., Kawai, H., Shimosaka, A., Kami, M., Tanosaki, R., Wakasugi, H., Saito, S., Takaue, Y., & Kakizoe, T. (2004). Freeze-Thawing Procedures Have No Influence on the Phenotypic and Functional Development of Dendritic Cells Generated from Peripheral Blood CD14+ Monocytes. Journal of Immunotherapy, 27(1), 27-35. https://doi.org/10.1097/00002371-200401000-00003