Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis

Hiroyuki Nakagawa, Kokichi Sugano, Takuma Fujii, Kaneyuki Kubushiro, Katsumi Tsukazaki, Shiro Nozawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. Materials and Methods: Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. Results: In 95 samples of cervical dysplasia, HPV infection was detected in 98.9% (94 out of 95), multiple HPV infection was detected in 38.3% (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2% (16 out of 19) and 55.6% (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6% (11 out of 16) in SCC and HPV 18 occupied 100% (5 out of 5) in adenocarcinoma. Conclusion: Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.

Original languageEnglish
Pages (from-to)1655-1660
Number of pages6
JournalAnticancer Research
Volume22
Issue number3
Publication statusPublished - 2002

Fingerprint

Papillomaviridae
Uterine Cervical Dysplasia
Polymerase Chain Reaction
DNA
Virus Diseases
Nucleic Acid Conformation
Squamous Cell Carcinoma
Adenocarcinoma
Virulence
Carcinoma in Situ
Infection

Keywords

  • Cervical carcinoma
  • Genotyping
  • Human papilloma virus
  • Multiple infection
  • Polymerase chain reacton
  • Single-strand DNA conformation polymorphism

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nakagawa, H., Sugano, K., Fujii, T., Kubushiro, K., Tsukazaki, K., & Nozawa, S. (2002). Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis. Anticancer Research, 22(3), 1655-1660.

Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis. / Nakagawa, Hiroyuki; Sugano, Kokichi; Fujii, Takuma; Kubushiro, Kaneyuki; Tsukazaki, Katsumi; Nozawa, Shiro.

In: Anticancer Research, Vol. 22, No. 3, 2002, p. 1655-1660.

Research output: Contribution to journalArticle

Nakagawa, H, Sugano, K, Fujii, T, Kubushiro, K, Tsukazaki, K & Nozawa, S 2002, 'Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis', Anticancer Research, vol. 22, no. 3, pp. 1655-1660.
Nakagawa, Hiroyuki ; Sugano, Kokichi ; Fujii, Takuma ; Kubushiro, Kaneyuki ; Tsukazaki, Katsumi ; Nozawa, Shiro. / Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis. In: Anticancer Research. 2002 ; Vol. 22, No. 3. pp. 1655-1660.
@article{304e111819814f108397c459765604aa,
title = "Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis",
abstract = "Background: To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. Materials and Methods: Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. Results: In 95 samples of cervical dysplasia, HPV infection was detected in 98.9{\%} (94 out of 95), multiple HPV infection was detected in 38.3{\%} (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2{\%} (16 out of 19) and 55.6{\%} (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6{\%} (11 out of 16) in SCC and HPV 18 occupied 100{\%} (5 out of 5) in adenocarcinoma. Conclusion: Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.",
keywords = "Cervical carcinoma, Genotyping, Human papilloma virus, Multiple infection, Polymerase chain reacton, Single-strand DNA conformation polymorphism",
author = "Hiroyuki Nakagawa and Kokichi Sugano and Takuma Fujii and Kaneyuki Kubushiro and Katsumi Tsukazaki and Shiro Nozawa",
year = "2002",
language = "English",
volume = "22",
pages = "1655--1660",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3",

}

TY - JOUR

T1 - Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis

AU - Nakagawa, Hiroyuki

AU - Sugano, Kokichi

AU - Fujii, Takuma

AU - Kubushiro, Kaneyuki

AU - Tsukazaki, Katsumi

AU - Nozawa, Shiro

PY - 2002

Y1 - 2002

N2 - Background: To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. Materials and Methods: Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. Results: In 95 samples of cervical dysplasia, HPV infection was detected in 98.9% (94 out of 95), multiple HPV infection was detected in 38.3% (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2% (16 out of 19) and 55.6% (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6% (11 out of 16) in SCC and HPV 18 occupied 100% (5 out of 5) in adenocarcinoma. Conclusion: Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.

AB - Background: To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. Materials and Methods: Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. Results: In 95 samples of cervical dysplasia, HPV infection was detected in 98.9% (94 out of 95), multiple HPV infection was detected in 38.3% (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2% (16 out of 19) and 55.6% (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6% (11 out of 16) in SCC and HPV 18 occupied 100% (5 out of 5) in adenocarcinoma. Conclusion: Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.

KW - Cervical carcinoma

KW - Genotyping

KW - Human papilloma virus

KW - Multiple infection

KW - Polymerase chain reacton

KW - Single-strand DNA conformation polymorphism

UR - http://www.scopus.com/inward/record.url?scp=0036323469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036323469&partnerID=8YFLogxK

M3 - Article

C2 - 12168850

AN - SCOPUS:0036323469

VL - 22

SP - 1655

EP - 1660

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 3

ER -