Frequent mutations of genes encoding vacuolar H + -ATPase components in granular cell tumors

Masaya Sekimizu, Akihiko Yoshida, Sachiyo Mitani, Naofumi Asano, Makoto Hirata, Takashi Kubo, Fumito Yamazaki, Hiromi Sakamoto, Mamoru Kato, Naohiro Makise, Taisuke Mori, Naoya Yamazaki, Shigeki Sekine, Ichiro Oda, Shun ichi Watanabe, Hiroaki Hiraga, Tsukasa Yonemoto, Teruya Kawamoto, Norifumi Naka, Yuki FunauchiYoshihiro Nishida, Kanya Honoki, Hirotaka Kawano, Hiroyuki Tsuchiya, Toshiyuki Kunisada, Koichi Matsuda, Katsunori Inagaki, Akira Kawai, Hitoshi Ichikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Granular cell tumors (GCTs) are rare mesenchymal tumors that exhibit a characteristic morphology and a finely granular cytoplasm. The genetic alterations responsible for GCT tumorigenesis had been unknown until recently, when loss-of-function mutations of ATP6AP1 and ATP6AP2 were described. Thus, we performed whole-exome sequencing, RNA sequencing, and targeted sequencing of 51 GCT samples. From these genomic analyses, we identified mutations in genes encoding vacuolar H + -ATPase (V-ATPase) components, including ATP6AP1 and ATP6AP2, in 33 (65%) GCTs. ATP6AP1 and ATP6AP2 mutations were found in 23 (45%) and 2 (4%) samples, respectively, and all were truncating or splice site mutations. In addition, seven other genes encoding V-ATPase components were also mutated, and three mutations in ATP6V0C occurred on the same amino acid (isoleucine 136). These V-ATPase component gene mutations were mutually exclusive, with one exception. These results suggest that V-ATPase function is impaired in GCTs not only by loss-of-function mutations of ATP6AP1 and ATP6AP2 but also through mutations of other subunits. Our findings provide additional support for the hypothesis that V-ATPase dysfunction promotes GCT tumorigenesis.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalGenes Chromosomes and Cancer
Volume58
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

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Vacuolar Proton-Translocating ATPases
Granular Cell Tumor
Mutation
Genes
Carcinogenesis
Gene Components
Exome
RNA Sequence Analysis
Isoleucine
Cytoplasm
Amino Acids

Keywords

  • genomic profiling
  • granular cell tumor
  • V-ATPase

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Frequent mutations of genes encoding vacuolar H + -ATPase components in granular cell tumors . / Sekimizu, Masaya; Yoshida, Akihiko; Mitani, Sachiyo; Asano, Naofumi; Hirata, Makoto; Kubo, Takashi; Yamazaki, Fumito; Sakamoto, Hiromi; Kato, Mamoru; Makise, Naohiro; Mori, Taisuke; Yamazaki, Naoya; Sekine, Shigeki; Oda, Ichiro; Watanabe, Shun ichi; Hiraga, Hiroaki; Yonemoto, Tsukasa; Kawamoto, Teruya; Naka, Norifumi; Funauchi, Yuki; Nishida, Yoshihiro; Honoki, Kanya; Kawano, Hirotaka; Tsuchiya, Hiroyuki; Kunisada, Toshiyuki; Matsuda, Koichi; Inagaki, Katsunori; Kawai, Akira; Ichikawa, Hitoshi.

In: Genes Chromosomes and Cancer, Vol. 58, No. 6, 01.06.2019, p. 373-380.

Research output: Contribution to journalArticle

Sekimizu, M, Yoshida, A, Mitani, S, Asano, N, Hirata, M, Kubo, T, Yamazaki, F, Sakamoto, H, Kato, M, Makise, N, Mori, T, Yamazaki, N, Sekine, S, Oda, I, Watanabe, SI, Hiraga, H, Yonemoto, T, Kawamoto, T, Naka, N, Funauchi, Y, Nishida, Y, Honoki, K, Kawano, H, Tsuchiya, H, Kunisada, T, Matsuda, K, Inagaki, K, Kawai, A & Ichikawa, H 2019, ' Frequent mutations of genes encoding vacuolar H + -ATPase components in granular cell tumors ', Genes Chromosomes and Cancer, vol. 58, no. 6, pp. 373-380. https://doi.org/10.1002/gcc.22727
Sekimizu, Masaya ; Yoshida, Akihiko ; Mitani, Sachiyo ; Asano, Naofumi ; Hirata, Makoto ; Kubo, Takashi ; Yamazaki, Fumito ; Sakamoto, Hiromi ; Kato, Mamoru ; Makise, Naohiro ; Mori, Taisuke ; Yamazaki, Naoya ; Sekine, Shigeki ; Oda, Ichiro ; Watanabe, Shun ichi ; Hiraga, Hiroaki ; Yonemoto, Tsukasa ; Kawamoto, Teruya ; Naka, Norifumi ; Funauchi, Yuki ; Nishida, Yoshihiro ; Honoki, Kanya ; Kawano, Hirotaka ; Tsuchiya, Hiroyuki ; Kunisada, Toshiyuki ; Matsuda, Koichi ; Inagaki, Katsunori ; Kawai, Akira ; Ichikawa, Hitoshi. / Frequent mutations of genes encoding vacuolar H + -ATPase components in granular cell tumors In: Genes Chromosomes and Cancer. 2019 ; Vol. 58, No. 6. pp. 373-380.
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AU - Yoshida, Akihiko

AU - Mitani, Sachiyo

AU - Asano, Naofumi

AU - Hirata, Makoto

AU - Kubo, Takashi

AU - Yamazaki, Fumito

AU - Sakamoto, Hiromi

AU - Kato, Mamoru

AU - Makise, Naohiro

AU - Mori, Taisuke

AU - Yamazaki, Naoya

AU - Sekine, Shigeki

AU - Oda, Ichiro

AU - Watanabe, Shun ichi

AU - Hiraga, Hiroaki

AU - Yonemoto, Tsukasa

AU - Kawamoto, Teruya

AU - Naka, Norifumi

AU - Funauchi, Yuki

AU - Nishida, Yoshihiro

AU - Honoki, Kanya

AU - Kawano, Hirotaka

AU - Tsuchiya, Hiroyuki

AU - Kunisada, Toshiyuki

AU - Matsuda, Koichi

AU - Inagaki, Katsunori

AU - Kawai, Akira

AU - Ichikawa, Hitoshi

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