FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan

Hiroaki Kubota, Yoshifumi Uwamino, Mari Matsui, Tsuyoshi Sekizuka, Yasunori Suzuki, Rumi Okuno, Yumi Uchitani, Tsukasa Ariyoshi, Wataru Aoki, Satowa Suzuki, Makoto Kuroda, Takayuki Shinkai, Keiko Yokoyama, Kenji Sadamasu, Takeru Funakoshi, Mitsuru Murata, Naoki Hasegawa, Satoshi Iwata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the ‘Big Five’ carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase. Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced. Results: A novel carbapenemase gene, bla FRI-4 , encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with bla FRI-4 showed reduced carbapenem susceptibility. A complete sequence of the bla FRI-4 -carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that bla FRI-4 and the surrounding region (18.7 kb) were duplicated. Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.

Original languageEnglish
Pages (from-to)2969-2972
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume73
Issue number11
DOIs
Publication statusPublished - 2018

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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    Kubota, H., Uwamino, Y., Matsui, M., Sekizuka, T., Suzuki, Y., Okuno, R., Uchitani, Y., Ariyoshi, T., Aoki, W., Suzuki, S., Kuroda, M., Shinkai, T., Yokoyama, K., Sadamasu, K., Funakoshi, T., Murata, M., Hasegawa, N., & Iwata, S. (2018). FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan. Journal of Antimicrobial Chemotherapy, 73(11), 2969-2972. https://doi.org/10.1093/jac/dky291