FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan

Hiroaki Kubota, Yoshifumi Uwamino, Mari Matsui, Tsuyoshi Sekizuka, Yasunori Suzuki, Rumi Okuno, Yumi Uchitani, Tsukasa Ariyoshi, Wataru Aoki, Satowa Suzuki, Makoto Kuroda, Takayuki Shinkai, Keiko Yokoyama, Kenji Sadamasu, Takeru Funakoshi, Mitsuru Murata, Naoki Hasegawa, Satoshi Iwata

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the 'Big Five' carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase.

Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced.

Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated.

Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.

Original languageEnglish
Pages (from-to)2969-2972
Number of pages4
JournalThe Journal of antimicrobial chemotherapy
Volume73
Issue number11
DOIs
Publication statusPublished - 2018 Nov 1

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Enterobacter cloacae
Tokyo
Japan
Plasmids
Carbapenems
Genes
Escherichia coli
carbapenemase
Amino Acid Sequence
Anti-Bacterial Agents
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan. / Kubota, Hiroaki; Uwamino, Yoshifumi; Matsui, Mari; Sekizuka, Tsuyoshi; Suzuki, Yasunori; Okuno, Rumi; Uchitani, Yumi; Ariyoshi, Tsukasa; Aoki, Wataru; Suzuki, Satowa; Kuroda, Makoto; Shinkai, Takayuki; Yokoyama, Keiko; Sadamasu, Kenji; Funakoshi, Takeru; Murata, Mitsuru; Hasegawa, Naoki; Iwata, Satoshi.

In: The Journal of antimicrobial chemotherapy, Vol. 73, No. 11, 01.11.2018, p. 2969-2972.

Research output: Contribution to journalArticle

Kubota, H, Uwamino, Y, Matsui, M, Sekizuka, T, Suzuki, Y, Okuno, R, Uchitani, Y, Ariyoshi, T, Aoki, W, Suzuki, S, Kuroda, M, Shinkai, T, Yokoyama, K, Sadamasu, K, Funakoshi, T, Murata, M, Hasegawa, N & Iwata, S 2018, 'FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan', The Journal of antimicrobial chemotherapy, vol. 73, no. 11, pp. 2969-2972. https://doi.org/10.1093/jac/dky291
Kubota, Hiroaki ; Uwamino, Yoshifumi ; Matsui, Mari ; Sekizuka, Tsuyoshi ; Suzuki, Yasunori ; Okuno, Rumi ; Uchitani, Yumi ; Ariyoshi, Tsukasa ; Aoki, Wataru ; Suzuki, Satowa ; Kuroda, Makoto ; Shinkai, Takayuki ; Yokoyama, Keiko ; Sadamasu, Kenji ; Funakoshi, Takeru ; Murata, Mitsuru ; Hasegawa, Naoki ; Iwata, Satoshi. / FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan. In: The Journal of antimicrobial chemotherapy. 2018 ; Vol. 73, No. 11. pp. 2969-2972.
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abstract = "Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the 'Big Five' carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase.Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced.Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2{\%} identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated.Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.",
author = "Hiroaki Kubota and Yoshifumi Uwamino and Mari Matsui and Tsuyoshi Sekizuka and Yasunori Suzuki and Rumi Okuno and Yumi Uchitani and Tsukasa Ariyoshi and Wataru Aoki and Satowa Suzuki and Makoto Kuroda and Takayuki Shinkai and Keiko Yokoyama and Kenji Sadamasu and Takeru Funakoshi and Mitsuru Murata and Naoki Hasegawa and Satoshi Iwata",
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T1 - FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan

AU - Kubota, Hiroaki

AU - Uwamino, Yoshifumi

AU - Matsui, Mari

AU - Sekizuka, Tsuyoshi

AU - Suzuki, Yasunori

AU - Okuno, Rumi

AU - Uchitani, Yumi

AU - Ariyoshi, Tsukasa

AU - Aoki, Wataru

AU - Suzuki, Satowa

AU - Kuroda, Makoto

AU - Shinkai, Takayuki

AU - Yokoyama, Keiko

AU - Sadamasu, Kenji

AU - Funakoshi, Takeru

AU - Murata, Mitsuru

AU - Hasegawa, Naoki

AU - Iwata, Satoshi

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the 'Big Five' carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase.Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced.Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated.Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.

AB - Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the 'Big Five' carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase.Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced.Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated.Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.

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