TY - JOUR
T1 - FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan
AU - Kubota, Hiroaki
AU - Uwamino, Yoshifumi
AU - Matsui, Mari
AU - Sekizuka, Tsuyoshi
AU - Suzuki, Yasunori
AU - Okuno, Rumi
AU - Uchitani, Yumi
AU - Ariyoshi, Tsukasa
AU - Aoki, Wataru
AU - Suzuki, Satowa
AU - Kuroda, Makoto
AU - Shinkai, Takayuki
AU - Yokoyama, Keiko
AU - Sadamasu, Kenji
AU - Funakoshi, Takeru
AU - Murata, Mitsuru
AU - Hasegawa, Naoki
AU - Iwata, Satoshi
N1 - Funding Information:
This work was supported by the Research Program on Emerging and Reemerging Infectious Diseases from the Japan Agency for Medical Research and Development, AMED (grant numbers: 17fk0108121j and 18fk0108048j), and by a grant for Research on Emerging and Re-emerging Infectious Diseases and Immunization from the Japanese Ministry of Health, Labour and Welfare (grant number: H28-Shinko Gyosei-Ippan-006).
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the ‘Big Five’ carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase. Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced. Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated. Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.
AB - Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the ‘Big Five’ carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase. Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced. Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated. Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.
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U2 - 10.1093/jac/dky291
DO - 10.1093/jac/dky291
M3 - Article
C2 - 30060114
AN - SCOPUS:85055207804
VL - 73
SP - 2969
EP - 2972
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 11
ER -