Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Takayuki Michikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Research output: Contribution to journalArticlepeer-review

Abstract

Multi-photon holographic patterned illumination using temporal focusing (TF) pulses provides 10 μm multi-spots with an axial confinement of 5 μm, enabling parallel photo-stimulation of multi-target cells in three dimensions. However, interference fringes and speckles degrade the uniformity of the spots, causing spatio-temporal fluctuations in photoactivation. Here, we demonstrate fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed (TM) TF pulses even when the multi-spots overlap spatially. The uniform patterned illumination enabled the expansion of the field-of-view of TM-TF microscopy by more than fivefold and enhanced the axial resolution to 1.3 μm.

Original languageEnglish
Article number042005
JournalApplied Physics Express
Volume15
Issue number4
DOIs
Publication statusPublished - 2022 Apr

Keywords

  • holographic patterned illumination
  • multiphoton microscopy
  • temporal focusing

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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