From the olfactory bulb to higher brain Centers

Genetic visualization of secondary olfactory pathways in zebrafish

Nobuhiko Miyasaka, Kozo Morimoto, Tatsuya Tsubokawa, Shin Ichi Higashijima, Hitoshi Okamoto, Yoshihiro Yoshihara

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

In the vertebrate olfactory system, odor information is represented as a topographic map in the olfactory bulb (OB). However, it remains unknown how this odor map is transferred from the OB to higher olfactory centers. Using genetic labeling techniques in zebrafish, we found that the OB output neurons, mitral cells (MCs), are heterogeneous with respect to transgene expression profiles and spatial distributions. Tracing MC axons at single-cell resolution revealed that (1) individual MCs send axons to multiple target regions in the forebrain; (2) MCs innervating the same glomerulus do not necessarily display the same axon trajectory; (3) MCs innervating distinct glomerular clusters tend to project axons to different, but partly overlapping, target regions; (4) MCs innervating the medial glomerular cluster directly and asymmetrically send axons to the right habenula. We propose that the topographic odor map in the OB is not maintained intact, but reorganized in higher olfactory centers. Moreover, our finding of asymmetric bulbo-habenular projection renders the olfactory system an attractive model for the studies of brain asymmetry and lateralized behaviors.

Original languageEnglish
Pages (from-to)4756-4767
Number of pages12
JournalJournal of Neuroscience
Volume29
Issue number15
DOIs
Publication statusPublished - 2009 Apr 15

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Olfactory Pathways
Olfactory Bulb
Zebrafish
Axons
Brain
Habenula
Genetic Techniques
Prosencephalon
Transgenes
Vertebrates
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

From the olfactory bulb to higher brain Centers : Genetic visualization of secondary olfactory pathways in zebrafish. / Miyasaka, Nobuhiko; Morimoto, Kozo; Tsubokawa, Tatsuya; Higashijima, Shin Ichi; Okamoto, Hitoshi; Yoshihara, Yoshihiro.

In: Journal of Neuroscience, Vol. 29, No. 15, 15.04.2009, p. 4756-4767.

Research output: Contribution to journalArticle

Miyasaka, Nobuhiko ; Morimoto, Kozo ; Tsubokawa, Tatsuya ; Higashijima, Shin Ichi ; Okamoto, Hitoshi ; Yoshihara, Yoshihiro. / From the olfactory bulb to higher brain Centers : Genetic visualization of secondary olfactory pathways in zebrafish. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 15. pp. 4756-4767.
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