Abstract
In the vertebrate olfactory system, odor information is represented as a topographic map in the olfactory bulb (OB). However, it remains unknown how this odor map is transferred from the OB to higher olfactory centers. Using genetic labeling techniques in zebrafish, we found that the OB output neurons, mitral cells (MCs), are heterogeneous with respect to transgene expression profiles and spatial distributions. Tracing MC axons at single-cell resolution revealed that (1) individual MCs send axons to multiple target regions in the forebrain; (2) MCs innervating the same glomerulus do not necessarily display the same axon trajectory; (3) MCs innervating distinct glomerular clusters tend to project axons to different, but partly overlapping, target regions; (4) MCs innervating the medial glomerular cluster directly and asymmetrically send axons to the right habenula. We propose that the topographic odor map in the OB is not maintained intact, but reorganized in higher olfactory centers. Moreover, our finding of asymmetric bulbo-habenular projection renders the olfactory system an attractive model for the studies of brain asymmetry and lateralized behaviors.
| Original language | English |
|---|---|
| Pages (from-to) | 4756-4767 |
| Number of pages | 12 |
| Journal | Journal of Neuroscience |
| Volume | 29 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - 2009 Apr 15 |
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ASJC Scopus subject areas
- Neuroscience(all)
- Medicine(all)
Cite this
From the olfactory bulb to higher brain Centers : Genetic visualization of secondary olfactory pathways in zebrafish. / Miyasaka, Nobuhiko; Morimoto, Kozo; Tsubokawa, Tatsuya; Higashijima, Shin Ichi; Okamoto, Hitoshi; Yoshihara, Yoshihiro.
In: Journal of Neuroscience, Vol. 29, No. 15, 15.04.2009, p. 4756-4767.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - From the olfactory bulb to higher brain Centers
T2 - Genetic visualization of secondary olfactory pathways in zebrafish
AU - Miyasaka, Nobuhiko
AU - Morimoto, Kozo
AU - Tsubokawa, Tatsuya
AU - Higashijima, Shin Ichi
AU - Okamoto, Hitoshi
AU - Yoshihara, Yoshihiro
PY - 2009/4/15
Y1 - 2009/4/15
N2 - In the vertebrate olfactory system, odor information is represented as a topographic map in the olfactory bulb (OB). However, it remains unknown how this odor map is transferred from the OB to higher olfactory centers. Using genetic labeling techniques in zebrafish, we found that the OB output neurons, mitral cells (MCs), are heterogeneous with respect to transgene expression profiles and spatial distributions. Tracing MC axons at single-cell resolution revealed that (1) individual MCs send axons to multiple target regions in the forebrain; (2) MCs innervating the same glomerulus do not necessarily display the same axon trajectory; (3) MCs innervating distinct glomerular clusters tend to project axons to different, but partly overlapping, target regions; (4) MCs innervating the medial glomerular cluster directly and asymmetrically send axons to the right habenula. We propose that the topographic odor map in the OB is not maintained intact, but reorganized in higher olfactory centers. Moreover, our finding of asymmetric bulbo-habenular projection renders the olfactory system an attractive model for the studies of brain asymmetry and lateralized behaviors.
AB - In the vertebrate olfactory system, odor information is represented as a topographic map in the olfactory bulb (OB). However, it remains unknown how this odor map is transferred from the OB to higher olfactory centers. Using genetic labeling techniques in zebrafish, we found that the OB output neurons, mitral cells (MCs), are heterogeneous with respect to transgene expression profiles and spatial distributions. Tracing MC axons at single-cell resolution revealed that (1) individual MCs send axons to multiple target regions in the forebrain; (2) MCs innervating the same glomerulus do not necessarily display the same axon trajectory; (3) MCs innervating distinct glomerular clusters tend to project axons to different, but partly overlapping, target regions; (4) MCs innervating the medial glomerular cluster directly and asymmetrically send axons to the right habenula. We propose that the topographic odor map in the OB is not maintained intact, but reorganized in higher olfactory centers. Moreover, our finding of asymmetric bulbo-habenular projection renders the olfactory system an attractive model for the studies of brain asymmetry and lateralized behaviors.
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UR - http://www.scopus.com/inward/citedby.url?scp=65549163920&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.0118-09.2009
DO - 10.1523/JNEUROSCI.0118-09.2009
M3 - Article
C2 - 19369545
AN - SCOPUS:65549163920
VL - 29
SP - 4756
EP - 4767
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 15
ER -