Function of fibrinogen γ-chain dodecapeptide-conjugated latex beads under flow

Shinji Takeoka, Yosuke Okamura, Yuji Teramura, Naohide Watanabe, Hidenori Suzuki, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In order to perform a fundamental study of platelet substitutes, novel particles that bound to activated platelets were prepared using two oligopeptides conjugated to latex beads. The oligopeptides were CHHLGGAKQAGDV (H12), which is a fibrinogen γ-chain carboxy-terminal sequence (γ 400-411), and CGGRGDF (RGD), which contains a fibrinogen α-chain sequence (α 95-98 RGDF). Both peptides contained an additional amino-terminal cysteine to enable conjugation. Human serum albumin was adsorbed onto the surface of latex beads (average diameter 1μm) and pyridyldisulfide groups were chemically introduced into the adsorbed protein. H12 or RGD peptides were then chemically linked to the modified surface protein via disulfide linkages. H12- or RGD-conjugated latex beads prepared in this way enhanced the in vitro thrombus formation of activated platelets on collagen-immobilized plates under flowing thrombocytopenic-imitation blood. Based on the result of flow cytometric analyses of agglutination, PAC-1 binding, antiP-selectin antibody binding, and annexin V binding, the H12-conjugated latex beads showed minimal interaction with non-activated platelets. These results indicate the excellent potential of H12-conjugated particles as a candidate for a platelet substitute.

Original languageEnglish
Pages (from-to)773-779
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume312
Issue number3
DOIs
Publication statusPublished - 2003 Dec 19

Keywords

  • Annexin V
  • Flow condition
  • Flow cytometry
  • H12
  • Latex beads
  • P-selectin
  • PAC-1
  • Platelet substitute
  • RGD
  • Thrombocytopenic-imitation blood

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Takeoka, S., Okamura, Y., Teramura, Y., Watanabe, N., Suzuki, H., Tsuchida, E., Handa, M., & Ikeda, Y. (2003). Function of fibrinogen γ-chain dodecapeptide-conjugated latex beads under flow. Biochemical and Biophysical Research Communications, 312(3), 773-779. https://doi.org/10.1016/j.bbrc.2003.10.184