Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation

Y. Morita-Hoshi, Y. Heike, M. Kawakami, T. Sugita, O. Miura, S. W. Kim, S. I. Mori, T. Fukuda, R. Tanosaki, K. Tobinai, Y. Takaue

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Abstract

In order to evaluate whether we could predict reactivation of CMV by monitoring the number of CMV-specific cytotoxic T-lymphocytes (CTL), tetramer analysis was performed in 37 patients who underwent hematopoietic stem cell transplantation (HSCT). The results disclosed that the mean number of CMV-specific CTL at day 30 did not differ among patients who developed CMV antigenemia (22/μl) and those who did not (12/μl). Serial tetramer analysis showed that 21% of the patients had >10/μl CMV-specific CTL at the first detection of CMV antigenemia and 67% of the patients had more than 10/μl CMV-specific CTL at the onset of CMV disease. Intracellular staining upon stimulation by CMV lysates and peptide in patients with CMV colitis revealed that both IFN-γ producing CD4+ and CD8+ lymphocytes were suppressed at the onset of CMV colitis (1.6 and 8/μl), which increased with recovery of the disease (19 and 47/μl). These data suggest that it is difficult to predict CMV reactivation solely by the number of CMV-specific CTL. We suggest that additional functional analysis by intracellular cytokine assay may be useful for immunomonitoring against CMV.

Original languageEnglish
Pages (from-to)515-521
Number of pages7
JournalBone Marrow Transplantation
Volume41
Issue number6
DOIs
Publication statusPublished - 2008 Mar

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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    Morita-Hoshi, Y., Heike, Y., Kawakami, M., Sugita, T., Miura, O., Kim, S. W., Mori, S. I., Fukuda, T., Tanosaki, R., Tobinai, K., & Takaue, Y. (2008). Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation. Bone Marrow Transplantation, 41(6), 515-521. https://doi.org/10.1038/sj.bmt.1705932