Abstract
In order to evaluate whether we could predict reactivation of CMV by monitoring the number of CMV-specific cytotoxic T-lymphocytes (CTL), tetramer analysis was performed in 37 patients who underwent hematopoietic stem cell transplantation (HSCT). The results disclosed that the mean number of CMV-specific CTL at day 30 did not differ among patients who developed CMV antigenemia (22/μl) and those who did not (12/μl). Serial tetramer analysis showed that 21% of the patients had >10/μl CMV-specific CTL at the first detection of CMV antigenemia and 67% of the patients had more than 10/μl CMV-specific CTL at the onset of CMV disease. Intracellular staining upon stimulation by CMV lysates and peptide in patients with CMV colitis revealed that both IFN-γ producing CD4+ and CD8+ lymphocytes were suppressed at the onset of CMV colitis (1.6 and 8/μl), which increased with recovery of the disease (19 and 47/μl). These data suggest that it is difficult to predict CMV reactivation solely by the number of CMV-specific CTL. We suggest that additional functional analysis by intracellular cytokine assay may be useful for immunomonitoring against CMV.
| Original language | English |
|---|---|
| Pages (from-to) | 515-521 |
| Number of pages | 7 |
| Journal | Bone Marrow Transplantation |
| Volume | 41 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2008 Mar |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
- Transplantation
Cite this
Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation. / Morita-Hoshi, Y.; Heike, Y.; Kawakami, M.; Sugita, T.; Miura, O.; Kim, S. W.; Mori, S. I.; Fukuda, T.; Tanosaki, Ryuji; Tobinai, K.; Takaue, Y.
In: Bone Marrow Transplantation, Vol. 41, No. 6, 03.2008, p. 515-521.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation
AU - Morita-Hoshi, Y.
AU - Heike, Y.
AU - Kawakami, M.
AU - Sugita, T.
AU - Miura, O.
AU - Kim, S. W.
AU - Mori, S. I.
AU - Fukuda, T.
AU - Tanosaki, Ryuji
AU - Tobinai, K.
AU - Takaue, Y.
PY - 2008/3
Y1 - 2008/3
N2 - In order to evaluate whether we could predict reactivation of CMV by monitoring the number of CMV-specific cytotoxic T-lymphocytes (CTL), tetramer analysis was performed in 37 patients who underwent hematopoietic stem cell transplantation (HSCT). The results disclosed that the mean number of CMV-specific CTL at day 30 did not differ among patients who developed CMV antigenemia (22/μl) and those who did not (12/μl). Serial tetramer analysis showed that 21% of the patients had >10/μl CMV-specific CTL at the first detection of CMV antigenemia and 67% of the patients had more than 10/μl CMV-specific CTL at the onset of CMV disease. Intracellular staining upon stimulation by CMV lysates and peptide in patients with CMV colitis revealed that both IFN-γ producing CD4+ and CD8+ lymphocytes were suppressed at the onset of CMV colitis (1.6 and 8/μl), which increased with recovery of the disease (19 and 47/μl). These data suggest that it is difficult to predict CMV reactivation solely by the number of CMV-specific CTL. We suggest that additional functional analysis by intracellular cytokine assay may be useful for immunomonitoring against CMV.
AB - In order to evaluate whether we could predict reactivation of CMV by monitoring the number of CMV-specific cytotoxic T-lymphocytes (CTL), tetramer analysis was performed in 37 patients who underwent hematopoietic stem cell transplantation (HSCT). The results disclosed that the mean number of CMV-specific CTL at day 30 did not differ among patients who developed CMV antigenemia (22/μl) and those who did not (12/μl). Serial tetramer analysis showed that 21% of the patients had >10/μl CMV-specific CTL at the first detection of CMV antigenemia and 67% of the patients had more than 10/μl CMV-specific CTL at the onset of CMV disease. Intracellular staining upon stimulation by CMV lysates and peptide in patients with CMV colitis revealed that both IFN-γ producing CD4+ and CD8+ lymphocytes were suppressed at the onset of CMV colitis (1.6 and 8/μl), which increased with recovery of the disease (19 and 47/μl). These data suggest that it is difficult to predict CMV reactivation solely by the number of CMV-specific CTL. We suggest that additional functional analysis by intracellular cytokine assay may be useful for immunomonitoring against CMV.
UR - http://www.scopus.com/inward/record.url?scp=41549162925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41549162925&partnerID=8YFLogxK
U2 - 10.1038/sj.bmt.1705932
DO - 10.1038/sj.bmt.1705932
M3 - Article
C2 - 18026143
AN - SCOPUS:41549162925
VL - 41
SP - 515
EP - 521
JO - Bone Marrow Transplantation
JF - Bone Marrow Transplantation
SN - 0268-3369
IS - 6
ER -