Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation

Y. Morita-Hoshi, Y. Heike, M. Kawakami, T. Sugita, O. Miura, S. W. Kim, S. I. Mori, T. Fukuda, Ryuji Tanosaki, K. Tobinai, Y. Takaue

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Abstract

In order to evaluate whether we could predict reactivation of CMV by monitoring the number of CMV-specific cytotoxic T-lymphocytes (CTL), tetramer analysis was performed in 37 patients who underwent hematopoietic stem cell transplantation (HSCT). The results disclosed that the mean number of CMV-specific CTL at day 30 did not differ among patients who developed CMV antigenemia (22/μl) and those who did not (12/μl). Serial tetramer analysis showed that 21% of the patients had >10/μl CMV-specific CTL at the first detection of CMV antigenemia and 67% of the patients had more than 10/μl CMV-specific CTL at the onset of CMV disease. Intracellular staining upon stimulation by CMV lysates and peptide in patients with CMV colitis revealed that both IFN-γ producing CD4+ and CD8+ lymphocytes were suppressed at the onset of CMV colitis (1.6 and 8/μl), which increased with recovery of the disease (19 and 47/μl). These data suggest that it is difficult to predict CMV reactivation solely by the number of CMV-specific CTL. We suggest that additional functional analysis by intracellular cytokine assay may be useful for immunomonitoring against CMV.

Original languageEnglish
Pages (from-to)515-521
Number of pages7
JournalBone Marrow Transplantation
Volume41
Issue number6
DOIs
Publication statusPublished - 2008 Mar
Externally publishedYes

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Hematopoietic Stem Cell Transplantation
Cytotoxic T-Lymphocytes
Cytomegalovirus
T-Lymphocytes
Colitis
Lymphocytes
Staining and Labeling
Cytokines
Peptides

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation. / Morita-Hoshi, Y.; Heike, Y.; Kawakami, M.; Sugita, T.; Miura, O.; Kim, S. W.; Mori, S. I.; Fukuda, T.; Tanosaki, Ryuji; Tobinai, K.; Takaue, Y.

In: Bone Marrow Transplantation, Vol. 41, No. 6, 03.2008, p. 515-521.

Research output: Contribution to journalArticle

Morita-Hoshi, Y, Heike, Y, Kawakami, M, Sugita, T, Miura, O, Kim, SW, Mori, SI, Fukuda, T, Tanosaki, R, Tobinai, K & Takaue, Y 2008, 'Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation', Bone Marrow Transplantation, vol. 41, no. 6, pp. 515-521. https://doi.org/10.1038/sj.bmt.1705932
Morita-Hoshi, Y. ; Heike, Y. ; Kawakami, M. ; Sugita, T. ; Miura, O. ; Kim, S. W. ; Mori, S. I. ; Fukuda, T. ; Tanosaki, Ryuji ; Tobinai, K. ; Takaue, Y. / Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation. In: Bone Marrow Transplantation. 2008 ; Vol. 41, No. 6. pp. 515-521.
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