Functional analysis of transcriptional activity of cytosine and adenine (CA) repeats polymorphism in the estrogen receptor β gene

Kanako Ugai, Kazuhiro Nishimura, Katsumi Fukino, Tomonori Nakamura, Koichi Ueno

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Cytosine and adenine (CA) repeats polymorphism (D14S1026) iterating "cytosine and adenine" nucleotide motifs is one of the genomic microsatellites in intron 5 of the estrogen receptor β (ER β) gene (14q22-24). Relations between CA repeats polymorphism and several diseases have been shown. Although the relation between number of CA repeats and gene transcription has been actively studied using several genes, results have remained contradictory until this time. In this study, we examined the functional effects of CA repeats polymorphism on transcriptional activity based on our knowledge of the ER β gene. After preparing four types of reporter gene constructs containing 15, 18, 24 or 27 CA repeats, luciferase reporter gene assays were performed. Relative luciferase activities of these constructs were not significantly different from that of the no inserted vector and variation of CA repeats did not affect these activities. Our results indicate that CA repeats polymorphism might not only affect transcriptional activity but also other processes of gene expressions. Further studies are needed to clarify the specific functions of CA repeats polymorphism in the ER β gene.

Original languageEnglish
Pages (from-to)237-240
Number of pages4
JournalJournal of Toxicological Sciences
Volume33
Issue number2
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

Keywords

  • Estrogen receptor β
  • Microsatellite
  • Polymorphism
  • Transcription

ASJC Scopus subject areas

  • Toxicology

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