Functional and morphological analysis of rehydrated lyophilized human platelets

T. A. Takahashi, K. Nakai, N. Sato, S. Fujikawa, K. Tadokoro, T. Jyuji, M. Murata, Y. Ikeda

Research output: Contribution to journalArticlepeer-review

Abstract

We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

Original languageEnglish
Pages (from-to)637-640
Number of pages4
JournalJapanese Journal of Artificial Organs
Volume26
Issue number3
Publication statusPublished - 1997

Keywords

  • Blood substitutes
  • Glycoprotein 1b
  • Lyophilization
  • Platelets
  • Ristocetin- dependent aggregation

ASJC Scopus subject areas

  • Biophysics

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