Functional and morphological analysis of rehydrated lyophilized human platelets

T. A. Takahashi, K. Nakai, N. Sato, S. Fujikawa, K. Tadokoro, T. Jyuji, Mitsuru Murata, Y. Ikeda

Research output: Contribution to journalArticle

Abstract

We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

Original languageEnglish
Pages (from-to)637-640
Number of pages4
JournalJapanese Journal of Artificial Organs
Volume26
Issue number3
Publication statusPublished - 1997

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Blood Platelets
Ristocetin
Integrin beta3
P-Selectin
Freeze Drying
Membrane Glycoproteins
Platelet Activation
Bovine Serum Albumin
Collagen
Electrons

Keywords

  • Blood substitutes
  • Glycoprotein 1b
  • Lyophilization
  • Platelets
  • Ristocetin- dependent aggregation

ASJC Scopus subject areas

  • Biophysics

Cite this

Takahashi, T. A., Nakai, K., Sato, N., Fujikawa, S., Tadokoro, K., Jyuji, T., ... Ikeda, Y. (1997). Functional and morphological analysis of rehydrated lyophilized human platelets. Japanese Journal of Artificial Organs, 26(3), 637-640.

Functional and morphological analysis of rehydrated lyophilized human platelets. / Takahashi, T. A.; Nakai, K.; Sato, N.; Fujikawa, S.; Tadokoro, K.; Jyuji, T.; Murata, Mitsuru; Ikeda, Y.

In: Japanese Journal of Artificial Organs, Vol. 26, No. 3, 1997, p. 637-640.

Research output: Contribution to journalArticle

Takahashi, TA, Nakai, K, Sato, N, Fujikawa, S, Tadokoro, K, Jyuji, T, Murata, M & Ikeda, Y 1997, 'Functional and morphological analysis of rehydrated lyophilized human platelets', Japanese Journal of Artificial Organs, vol. 26, no. 3, pp. 637-640.
Takahashi TA, Nakai K, Sato N, Fujikawa S, Tadokoro K, Jyuji T et al. Functional and morphological analysis of rehydrated lyophilized human platelets. Japanese Journal of Artificial Organs. 1997;26(3):637-640.
Takahashi, T. A. ; Nakai, K. ; Sato, N. ; Fujikawa, S. ; Tadokoro, K. ; Jyuji, T. ; Murata, Mitsuru ; Ikeda, Y. / Functional and morphological analysis of rehydrated lyophilized human platelets. In: Japanese Journal of Artificial Organs. 1997 ; Vol. 26, No. 3. pp. 637-640.
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