Functional characterization of podocan, a member of a new class in the small leucine-rich repeat protein family

Ryoko Shimizu, Hiroyuki Sasamura, Mari Kuroda, Emi Kobayashi, Takao Saruta

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27 Citations (Scopus)

Abstract

An important component of the extracellular matrix is the group of non-collagenous proteins belonging to the small leucine-rich repeat (SLR) protein family. A new SLR protein, podocan, with structural characteristics different from the known classes of the SLR protein family has been identified recently from the kidney. In this study, we examined the functional characteristics of this SLR protein expressed in cultured cells. Podocan was clearly observed intracellularly and was also detectable in the supernatant. Treatment of the expressed protein with various glycoenzymes suggested that podocan is a glycoprotein containing N-linked oligosaccharides but not a classical proteoglycan. Moreover, podocan was found to bind type 1 collagen. Cells transfected with podocan showed reductions in cell growth and migration, concomitant with increased p21 expression. Podocan mRNA was detected by reverse transcription polymerase chain reaction not only in the kidney, but also in other tissues including the heart and vascular smooth muscle cells, suggesting that podocan may have a potential role in growth regulation in cardiovascular tissues.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalFEBS Letters
Volume563
Issue number1-3
DOIs
Publication statusPublished - 2004 Apr 9

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Keywords

  • Collagen binding
  • Growth
  • Leucine-rich repeat
  • Migration
  • Podocan

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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