TY - JOUR
T1 - Functional characterization of the promoter for the gene encoding murine CD34
AU - Yamaguchia, Yuji
AU - Tenen, Daniel G.
AU - Suda, Toshio
N1 - Funding Information:
We wish to thank Dr. S.J. Ackerman for searching the Ghosh transcription factor data base. We also thank Dr. M. Ishihara and Dr. T. Taniguchi for generously providing us with IRF-1 and IRF-2 expression vectors, and Dr. R. Hromas for providing us with MZF-1 expression vectors. This work was supported by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.
PY - 1997/2/7
Y1 - 1997/2/7
N2 - Since CD34 expression is restricted to the hematopoietic stem cells and decreases in differentiating cells, the analysis of the CD34 promoter is of interest to understand regulation of gene expression in stem cells. To characterize the cis-acting elements which control murine CD34 (mCD34) gene expression, we sought to clone, sequence, and functionally analyze the mCD34 promoter. An 80% decrease in promoter activity was obtained when sequences between -119 bp and -59 bp upstream of the transcriptional start site were deleted. We identified several DNA-protein complexes which correspond to functional segments defined by the linker-scanning mutants. These findings indicated the presence of the important regulatory element between bp -119 and -100, GGTTAAAAGTGAAGTAGGAA. Furthermore, from the result of functional promoter analysis in the DNase I hypersensitive site (HS) located within 5 kb upstream of the mCD34 gene, the presence of the enhancer region in the NcoI/PstI fragment of 5' upstream, -2.8 kb to -1.9 kb, has been identified. These data will provide useful information on the gene transfer using the CD34 promoter and enhancer.
AB - Since CD34 expression is restricted to the hematopoietic stem cells and decreases in differentiating cells, the analysis of the CD34 promoter is of interest to understand regulation of gene expression in stem cells. To characterize the cis-acting elements which control murine CD34 (mCD34) gene expression, we sought to clone, sequence, and functionally analyze the mCD34 promoter. An 80% decrease in promoter activity was obtained when sequences between -119 bp and -59 bp upstream of the transcriptional start site were deleted. We identified several DNA-protein complexes which correspond to functional segments defined by the linker-scanning mutants. These findings indicated the presence of the important regulatory element between bp -119 and -100, GGTTAAAAGTGAAGTAGGAA. Furthermore, from the result of functional promoter analysis in the DNase I hypersensitive site (HS) located within 5 kb upstream of the mCD34 gene, the presence of the enhancer region in the NcoI/PstI fragment of 5' upstream, -2.8 kb to -1.9 kb, has been identified. These data will provide useful information on the gene transfer using the CD34 promoter and enhancer.
KW - CD34
KW - M1 cell
KW - enhancer region
KW - luciferase
KW - murine
KW - promoter region
KW - transcription initiation
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U2 - 10.1016/S0167-4781(96)00205-9
DO - 10.1016/S0167-4781(96)00205-9
M3 - Article
C2 - 9048883
AN - SCOPUS:0031056573
SN - 0167-4781
VL - 1350
SP - 141
EP - 146
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 2
ER -