Functional cloning and characterization of a multidrug efflux pump, MexHI-OpmD, from a Pseudomonas aeruginosa mutant

Hiroshi Sekiya, Takehiko Mima, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.

Original languageEnglish
Pages (from-to)2990-2992
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume47
Issue number9
DOIs
Publication statusPublished - 2003 Sep 1
Externally publishedYes

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Pseudomonas aeruginosa
Organism Cloning
Ethidium
Acriflavine
Genes
Norfloxacin
Plasmids
DNA
Pharmaceutical Preparations
rhodamine 6G

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Functional cloning and characterization of a multidrug efflux pump, MexHI-OpmD, from a Pseudomonas aeruginosa mutant. / Sekiya, Hiroshi; Mima, Takehiko; Morita, Yuji; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Antimicrobial Agents and Chemotherapy, Vol. 47, No. 9, 01.09.2003, p. 2990-2992.

Research output: Contribution to journalArticle

Sekiya, Hiroshi ; Mima, Takehiko ; Morita, Yuji ; Kuroda, Teruo ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Functional cloning and characterization of a multidrug efflux pump, MexHI-OpmD, from a Pseudomonas aeruginosa mutant. In: Antimicrobial Agents and Chemotherapy. 2003 ; Vol. 47, No. 9. pp. 2990-2992.
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