Functional comparison of transactivation by human retrovirus rev and rex genes

H. Sakai, H. Siomi, H. Shida, R. Shibata, T. Kiyomasu, A. Adachi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The effect of rev-responsive element deletion on human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) gene expression was examined. The phenotypes of HIV-1 and HIV-2 provirus DNAs lacking the rev-responsive element, as determined by transfection experiments, were indistinguishable from those of virus DNAs carrying rev gene mutations. By using rev-responsive elements derived from these two viruses, we developed two monitoring systems to evaluate the functionality of HIV-1 rev, HIV-2 rev, and human T-lymphotropic virus type I rex. In both systems, HIV-1 rev and human T-lymphotropic virus type I rex transactivated HIV-2 very efficiently. On the contrary, HIV-2 rev and human T-lymphotropic virus type I rex were poor activators of HIV-1. No functional replacement of rex by HIV-2 rev was observed.

Original languageEnglish
Pages (from-to)5833-5839
Number of pages7
JournalJournal of Virology
Volume64
Issue number12
Publication statusPublished - 1990 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Sakai, H., Siomi, H., Shida, H., Shibata, R., Kiyomasu, T., & Adachi, A. (1990). Functional comparison of transactivation by human retrovirus rev and rex genes. Journal of Virology, 64(12), 5833-5839.