Functional comparison of transactivation by human retrovirus rev and rex genes

Hiroyuki Sakai, Haruhiko Siomi, Hisatoshi Shida, Riri Shibata, Takahiro Kiyomasu, Akio Adach

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The effect of rev-responsive element deletion on human immunodeficiency virus type 1 (HIV-1) type 2 (HIV-2) gene expression was examined. The phenotypes of HIV-1 and HIV-2 provirus DNAs lacking the rev-responsive element, as determined by transfection experiments, were indistinguishable from those of virus DNAs carrying rev gene mutations. By using rev-responsive elements derived from these two viruses, we developed two monitoring systems to evaluate the functionality of HIV-1 rev, HIV-2 rev, and human T-Iymphotropic virus type I rex. In both systems, HIV-1 rev and human T-lymphotropic virus type I rex transactivated HIV-2 very efficiently. On the contrary, HIV-2 rev T-lymphotropic virus type I rex were poor activators of HIV-1. No functional replacement of rex by HIV-2 rev was observed.

Original languageEnglish
Pages (from-to)5833-5839
Number of pages7
JournalJournal of Virology
Volume64
Issue number12
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

rev Genes
Retroviridae
pX Genes
transcriptional activation
Human immunodeficiency virus 1
Transcriptional Activation
HIV-1
HIV-2
env Genes
genes
Viruses
viruses
proviruses
Proviruses
DNA viruses
DNA Viruses
transfection
Transfection

ASJC Scopus subject areas

  • Immunology

Cite this

Sakai, H., Siomi, H., Shida, H., Shibata, R., Kiyomasu, T., & Adach, A. (1990). Functional comparison of transactivation by human retrovirus rev and rex genes. Journal of Virology, 64(12), 5833-5839.

Functional comparison of transactivation by human retrovirus rev and rex genes. / Sakai, Hiroyuki; Siomi, Haruhiko; Shida, Hisatoshi; Shibata, Riri; Kiyomasu, Takahiro; Adach, Akio.

In: Journal of Virology, Vol. 64, No. 12, 1990, p. 5833-5839.

Research output: Contribution to journalArticle

Sakai, H, Siomi, H, Shida, H, Shibata, R, Kiyomasu, T & Adach, A 1990, 'Functional comparison of transactivation by human retrovirus rev and rex genes', Journal of Virology, vol. 64, no. 12, pp. 5833-5839.
Sakai H, Siomi H, Shida H, Shibata R, Kiyomasu T, Adach A. Functional comparison of transactivation by human retrovirus rev and rex genes. Journal of Virology. 1990;64(12):5833-5839.
Sakai, Hiroyuki ; Siomi, Haruhiko ; Shida, Hisatoshi ; Shibata, Riri ; Kiyomasu, Takahiro ; Adach, Akio. / Functional comparison of transactivation by human retrovirus rev and rex genes. In: Journal of Virology. 1990 ; Vol. 64, No. 12. pp. 5833-5839.
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