Functional domains of ZFP809 essential for nuclear localization and gene silencing

Yu Ichida, Yuko Utsunomiya, Toru Yasuda, Kazuhiko Nakabayashi, Toshinori Sato, Masafumi Onodera

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Zinc finger protein 809 (ZFP809) is a member of the Kruppel-associated box-containing zinc finger protein (KRAB-ZFP) family, and is highly expressed in mouse immature cells. ZFP809 is known to inhibit the expression of transduced genes driven by Moloney murine leukemia virus (MoMLV)-typed retroviral vectors by binding to the primer binding site (PBS) located downstream of the MLV-long terminal repeat (LTR) of the vectors and recruiting protein complexes that introduce epigenetic silencing marks such as histone modifications and DNA methylation at the MLV-LTR. However, it remains undetermined what domains of ZFP809 among the KRAB domain at N-terminus and the seven zinc fingers are critical for gene silencing. In this study, we assessed subcellular localization, gene silencing ability, and binding ability to the PBS of a series of truncated and mutated ZFP809 proteins. We revealed the essential role of the KRAB A box for all functions assessed, together with the accessory roles of a subset of zinc fingers. Our data also suggest that interaction between KAP1 and the KRAB A box of ZFP809 is critical in KAP1-dependent control of gene silencing for ZFP809 targets.

Original languageEnglish
Article numbere0139274
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - 2015 Sep 29

Fingerprint

zinc finger motif
Zinc Fingers
Gene Silencing
gene silencing
Zinc
Genes
Proteins
proteins
terminal repeat sequences
Terminal Repeat Sequences
binding sites
Histone Code
Binding Sites
Murine leukemia virus
retroviral vectors
Moloney murine leukemia virus
Accessories
binding capacity
DNA methylation
DNA Methylation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ichida, Y., Utsunomiya, Y., Yasuda, T., Nakabayashi, K., Sato, T., & Onodera, M. (2015). Functional domains of ZFP809 essential for nuclear localization and gene silencing. PLoS One, 10(9), [e0139274]. https://doi.org/10.1371/journal.pone.0139274

Functional domains of ZFP809 essential for nuclear localization and gene silencing. / Ichida, Yu; Utsunomiya, Yuko; Yasuda, Toru; Nakabayashi, Kazuhiko; Sato, Toshinori; Onodera, Masafumi.

In: PLoS One, Vol. 10, No. 9, e0139274, 29.09.2015.

Research output: Contribution to journalArticle

Ichida, Y, Utsunomiya, Y, Yasuda, T, Nakabayashi, K, Sato, T & Onodera, M 2015, 'Functional domains of ZFP809 essential for nuclear localization and gene silencing', PLoS One, vol. 10, no. 9, e0139274. https://doi.org/10.1371/journal.pone.0139274
Ichida, Yu ; Utsunomiya, Yuko ; Yasuda, Toru ; Nakabayashi, Kazuhiko ; Sato, Toshinori ; Onodera, Masafumi. / Functional domains of ZFP809 essential for nuclear localization and gene silencing. In: PLoS One. 2015 ; Vol. 10, No. 9.
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