Functional domains of ZFP809 essential for nuclear localization and gene silencing

Yu Ichida, Yuko Utsunomiya, Toru Yasuda, Kazuhiko Nakabayashi, Toshinori Sato, Masafumi Onodera

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3 Citations (Scopus)

Abstract

Zinc finger protein 809 (ZFP809) is a member of the Kruppel-associated box-containing zinc finger protein (KRAB-ZFP) family, and is highly expressed in mouse immature cells. ZFP809 is known to inhibit the expression of transduced genes driven by Moloney murine leukemia virus (MoMLV)-typed retroviral vectors by binding to the primer binding site (PBS) located downstream of the MLV-long terminal repeat (LTR) of the vectors and recruiting protein complexes that introduce epigenetic silencing marks such as histone modifications and DNA methylation at the MLV-LTR. However, it remains undetermined what domains of ZFP809 among the KRAB domain at N-terminus and the seven zinc fingers are critical for gene silencing. In this study, we assessed subcellular localization, gene silencing ability, and binding ability to the PBS of a series of truncated and mutated ZFP809 proteins. We revealed the essential role of the KRAB A box for all functions assessed, together with the accessory roles of a subset of zinc fingers. Our data also suggest that interaction between KAP1 and the KRAB A box of ZFP809 is critical in KAP1-dependent control of gene silencing for ZFP809 targets.

Original languageEnglish
Article numbere0139274
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - 2015 Sep 29

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ichida, Y., Utsunomiya, Y., Yasuda, T., Nakabayashi, K., Sato, T., & Onodera, M. (2015). Functional domains of ZFP809 essential for nuclear localization and gene silencing. PLoS One, 10(9), [e0139274]. https://doi.org/10.1371/journal.pone.0139274