Functional dynamics of cell surface membrane proteins

Noritaka Nishida, Masanori Osawa, Koh Takeuchi, Shunsuke Imai, Pavlos Stampoulis, Yutaka Kofuku, Takumi Ueda, Ichio Shimada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cell surface receptors are integral membrane proteins that receive external stimuli, and transmit signals across plasma membranes. In the conventional view of receptor activation, ligand binding to the extracellular side of the receptor induces conformational changes, which convert the structure of the receptor into an active conformation. However, recent NMR studies of cell surface membrane proteins have revealed that their structures are more dynamic than previously envisioned, and they fluctuate between multiple conformations in an equilibrium on various timescales. In addition, NMR analyses, along with biochemical and cell biological experiments indicated that such dynamical properties are critical for the proper functions of the receptors. In this review, we will describe several NMR studies that revealed direct linkage between the structural dynamics and the functions of the cell surface membrane proteins, such as G-protein coupled receptors (GPCRs), ion channels, membrane transporters, and cell adhesion molecules.

Original languageEnglish
Pages (from-to)86-96
Number of pages11
JournalJournal of Magnetic Resonance
Volume241
Issue number1
DOIs
Publication statusPublished - 2014 Apr
Externally publishedYes

Keywords

  • Cell surface receptors
  • Dynamics
  • Membrane proteins
  • NMR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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  • Cite this

    Nishida, N., Osawa, M., Takeuchi, K., Imai, S., Stampoulis, P., Kofuku, Y., Ueda, T., & Shimada, I. (2014). Functional dynamics of cell surface membrane proteins. Journal of Magnetic Resonance, 241(1), 86-96. https://doi.org/10.1016/j.jmr.2013.11.007