Functional dynamics of proteins revealed by solution NMR

Masanori Osawa, Koh Takeuchi, Takumi Ueda, Noritaka Nishida, Ichio Shimada

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Solution NMR spectroscopy can analyze the dynamics of proteins on a wide range of timescales, from picoseconds to even days, in a site-specific manner, and thus its results are complementary to the detailed but largely static structural information obtained by X-ray crystallography. We review recent progresses in a variety of NMR techniques, including relaxation dispersion and paramagnetic relaxation enhancement (PRE), that permit the observation of the low-populated states, which had been 'invisible' with other techniques. In addition, we review how NMR spectroscopy can be used to elucidate functionally relevant protein dynamics.

Original languageEnglish
Pages (from-to)660-669
Number of pages10
JournalCurrent Opinion in Structural Biology
Volume22
Issue number5
DOIs
Publication statusPublished - 2012 Oct
Externally publishedYes

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Magnetic Resonance Spectroscopy
Relaxation Therapy
X Ray Crystallography
Proteins
Observation

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Functional dynamics of proteins revealed by solution NMR. / Osawa, Masanori; Takeuchi, Koh; Ueda, Takumi; Nishida, Noritaka; Shimada, Ichio.

In: Current Opinion in Structural Biology, Vol. 22, No. 5, 10.2012, p. 660-669.

Research output: Contribution to journalArticle

Osawa, Masanori ; Takeuchi, Koh ; Ueda, Takumi ; Nishida, Noritaka ; Shimada, Ichio. / Functional dynamics of proteins revealed by solution NMR. In: Current Opinion in Structural Biology. 2012 ; Vol. 22, No. 5. pp. 660-669.
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