Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells

Hashimoto Yukiya Hashimoto, Ozaki Junko Ozaki, Yasuhara Masato Yasuhara, Hori Ryohei Hori, Suga Shin-ichi Suga, Hiroshi Itoh, Nakao Kazuwa Nakao, Inui Ken-inchi Inui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of α-human atrial natriuretic peptide (α-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with α-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for α-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.

Original languageEnglish
Pages (from-to)443-445
Number of pages3
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume268
Issue number3
DOIs
Publication statusPublished - 1994 Aug 16
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor Receptors
LLC-PK1 Cells
Cyclic GMP
Epithelial Cells
C-Type Natriuretic Peptide
Kidney
Natriuretic Peptides
Peptide Receptors
Membranes
Atrial Natriuretic Factor

Keywords

  • ANP (atrial natriuretic peptide)
  • Guanylate cyclase
  • LLC-PK cell
  • Natriuretic peptide, C-type

ASJC Scopus subject areas

  • Pharmacology

Cite this

Yukiya Hashimoto, H., Junko Ozaki, O., Masato Yasuhara, Y., Ryohei Hori, H., Shin-ichi Suga, S., Itoh, H., ... Ken-inchi Inui, I. (1994). Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells. European Journal of Pharmacology: Molecular Pharmacology, 268(3), 443-445. https://doi.org/10.1016/0922-4106(94)90071-X

Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells. / Yukiya Hashimoto, Hashimoto; Junko Ozaki, Ozaki; Masato Yasuhara, Yasuhara; Ryohei Hori, Hori; Shin-ichi Suga, Suga; Itoh, Hiroshi; Kazuwa Nakao, Nakao; Ken-inchi Inui, Inui.

In: European Journal of Pharmacology: Molecular Pharmacology, Vol. 268, No. 3, 16.08.1994, p. 443-445.

Research output: Contribution to journalArticle

Yukiya Hashimoto, H, Junko Ozaki, O, Masato Yasuhara, Y, Ryohei Hori, H, Shin-ichi Suga, S, Itoh, H, Kazuwa Nakao, N & Ken-inchi Inui, I 1994, 'Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells', European Journal of Pharmacology: Molecular Pharmacology, vol. 268, no. 3, pp. 443-445. https://doi.org/10.1016/0922-4106(94)90071-X
Yukiya Hashimoto, Hashimoto ; Junko Ozaki, Ozaki ; Masato Yasuhara, Yasuhara ; Ryohei Hori, Hori ; Shin-ichi Suga, Suga ; Itoh, Hiroshi ; Kazuwa Nakao, Nakao ; Ken-inchi Inui, Inui. / Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells. In: European Journal of Pharmacology: Molecular Pharmacology. 1994 ; Vol. 268, No. 3. pp. 443-445.
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AU - Shin-ichi Suga, Suga

AU - Itoh, Hiroshi

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