TY - JOUR
T1 - Functional expression of ABCG2 transporter in human neural stem/progenitor cells
AU - Islam, Mohammed Omedul
AU - Kanemura, Yonehiro
AU - Tajria, Jesmin
AU - Mori, Hideki
AU - Kobayashi, Satoshi
AU - Hara, Masayuki
AU - Yamasaki, Mami
AU - Okano, Hideyuki
AU - Miyake, Jun
N1 - Funding Information:
We thank Dr. Chiaki Ban at Osaka National Hospital for providing human fetal tissues and Dr. Susan E. Bates of the National Cancer Institute, NIH, USA, for providing the anti-ABCG2 (MXR87405) antibody. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2005/5
Y1 - 2005/5
N2 - We have studied the expression, localization, and function of the ABCG2 transporter, a universal stem cell marker, at the protein level in human cultured neural stem/progenitor cells (hNSPCs) using immunoblotting, immunofluorescence, and ATPase assays. Human NSPCs were isolated from human fetal brain and propagated in vitro as neurospheres. Both the cells in neurospheres and single cells dissociated from neurospheres showed high levels of ABCG2, and about 63% of the cells in neurospheres were ABCG2-positive, similar to the proportion of nestin-positive cells, and in most cases the ABCG2 and nestin staining co-localized in the same cells. Both the three-dimensional structure of single hNSPCs stained with anti-ABCG2 antibodies and an examination using a biochemical marker for the plasma membrane indicated that ABCG2 was localized to the plasma membrane of hNSPCs. The ABCG2 expressed in hNSPCs had prazosin-sensitive ATP hydrolysis activity, and the ABCG2 level was sharply down-regulated during hNSPC differentiation. All these results suggested that ABCG2, was functionally expressed in hNSPCs. ABCG2 might play a significant role in maintaining human neural stem cells in an undifferentiated state and in protecting hNSPCs from xenobiotics or other toxic substances in vivo.
AB - We have studied the expression, localization, and function of the ABCG2 transporter, a universal stem cell marker, at the protein level in human cultured neural stem/progenitor cells (hNSPCs) using immunoblotting, immunofluorescence, and ATPase assays. Human NSPCs were isolated from human fetal brain and propagated in vitro as neurospheres. Both the cells in neurospheres and single cells dissociated from neurospheres showed high levels of ABCG2, and about 63% of the cells in neurospheres were ABCG2-positive, similar to the proportion of nestin-positive cells, and in most cases the ABCG2 and nestin staining co-localized in the same cells. Both the three-dimensional structure of single hNSPCs stained with anti-ABCG2 antibodies and an examination using a biochemical marker for the plasma membrane indicated that ABCG2 was localized to the plasma membrane of hNSPCs. The ABCG2 expressed in hNSPCs had prazosin-sensitive ATP hydrolysis activity, and the ABCG2 level was sharply down-regulated during hNSPC differentiation. All these results suggested that ABCG2, was functionally expressed in hNSPCs. ABCG2 might play a significant role in maintaining human neural stem cells in an undifferentiated state and in protecting hNSPCs from xenobiotics or other toxic substances in vivo.
KW - ABC transporter
KW - ABCG2
KW - Human neural stem/progenitor cells
UR - http://www.scopus.com/inward/record.url?scp=16244362765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16244362765&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2005.01.013
DO - 10.1016/j.neures.2005.01.013
M3 - Article
C2 - 15811555
AN - SCOPUS:16244362765
SN - 0168-0102
VL - 52
SP - 75
EP - 82
JO - Neuroscience Research
JF - Neuroscience Research
IS - 1
ER -