Functional expression of costimulatory molecule CD86 on epithelial cells in the inflamed colonic mucosa

A. Nakazawa, M. Watanabe, Takanori Kanai, T. Yajima, M. Yamazaki, Haruhiko Ogata, H. Ishii, M. Azuma, T. Hibi

Research output: Contribution to journalArticle

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Abstract

Background and Aims: Costimulatory signals are essential for T-cell activation. The aim of this study was to demonstrate expression of costimulatory molecules CD86 and CD80 in human colonic epithelial cells and assess their functional roles in the activation of T cells in inflamed colonic mucosa. Methods: CD86 and CD80 expression was assessed by immunohistochemistry of colonic mucosa, reverse-transcription polymerase chain reaction, and flow cytometric analysis of isolated colonic epithelial cells and cell lines. Costimulatory effect of CD86-expressing colonic epithelial cells on purified CD4+ T-cell proliferation was also assessed at suboptimal phytohemagglutinin stimulation. Results: CD86 and CD80 messenger RNA was detected in isolated colonic epithelial cells from normal and inflamed mucosa of patients with ulcerative colitis. Immunohistochemistry and flow cytometric analysis of colonic epithelial cells confirmed cell surface expression of CD86 protein in inflamed colonic mucosa. Cell surface expression of CD86 protein was increased in the colonic epithelial cell line HT29-18-N2 after interferon gamma stimulation. Purified CD4+ T cells proliferated in response to suboptimal phytohemagglutinin costimulated with interferon gamma-stimulated HT2918-N2, and these proliferative responses were efficiently inhibited by the addition of anti-CD86 monoclonal antibody. Costimulatory effect of CD86-expressing colonic epithelial cells isolated from inflamed mucosa of ulcerative colitis was also demonstrated at suboptimal phytohemagglutinin stimulation in CD4+ T cells. Conclusions: Colonic epithelial cells may act as antigen-presenting cells, and contribute to the activation of T cells through costimulatory molecule CD86 expression in inflamed colonic mucosa.

Original languageEnglish
Pages (from-to)536-545
Number of pages10
JournalGastroenterology
Volume117
Issue number3
DOIs
Publication statusPublished - 1999

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Mucous Membrane
Epithelial Cells
T-Lymphocytes
Phytohemagglutinins
Ulcerative Colitis
Interferon-gamma
Immunohistochemistry
Cell Line
Antigen-Presenting Cells
Reverse Transcription
Proteins
Monoclonal Antibodies
Cell Proliferation
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Gastroenterology

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Functional expression of costimulatory molecule CD86 on epithelial cells in the inflamed colonic mucosa. / Nakazawa, A.; Watanabe, M.; Kanai, Takanori; Yajima, T.; Yamazaki, M.; Ogata, Haruhiko; Ishii, H.; Azuma, M.; Hibi, T.

In: Gastroenterology, Vol. 117, No. 3, 1999, p. 536-545.

Research output: Contribution to journalArticle

Nakazawa, A. ; Watanabe, M. ; Kanai, Takanori ; Yajima, T. ; Yamazaki, M. ; Ogata, Haruhiko ; Ishii, H. ; Azuma, M. ; Hibi, T. / Functional expression of costimulatory molecule CD86 on epithelial cells in the inflamed colonic mucosa. In: Gastroenterology. 1999 ; Vol. 117, No. 3. pp. 536-545.
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AU - Kanai, Takanori

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AU - Yamazaki, M.

AU - Ogata, Haruhiko

AU - Ishii, H.

AU - Azuma, M.

AU - Hibi, T.

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N2 - Background and Aims: Costimulatory signals are essential for T-cell activation. The aim of this study was to demonstrate expression of costimulatory molecules CD86 and CD80 in human colonic epithelial cells and assess their functional roles in the activation of T cells in inflamed colonic mucosa. Methods: CD86 and CD80 expression was assessed by immunohistochemistry of colonic mucosa, reverse-transcription polymerase chain reaction, and flow cytometric analysis of isolated colonic epithelial cells and cell lines. Costimulatory effect of CD86-expressing colonic epithelial cells on purified CD4+ T-cell proliferation was also assessed at suboptimal phytohemagglutinin stimulation. Results: CD86 and CD80 messenger RNA was detected in isolated colonic epithelial cells from normal and inflamed mucosa of patients with ulcerative colitis. Immunohistochemistry and flow cytometric analysis of colonic epithelial cells confirmed cell surface expression of CD86 protein in inflamed colonic mucosa. Cell surface expression of CD86 protein was increased in the colonic epithelial cell line HT29-18-N2 after interferon gamma stimulation. Purified CD4+ T cells proliferated in response to suboptimal phytohemagglutinin costimulated with interferon gamma-stimulated HT2918-N2, and these proliferative responses were efficiently inhibited by the addition of anti-CD86 monoclonal antibody. Costimulatory effect of CD86-expressing colonic epithelial cells isolated from inflamed mucosa of ulcerative colitis was also demonstrated at suboptimal phytohemagglutinin stimulation in CD4+ T cells. Conclusions: Colonic epithelial cells may act as antigen-presenting cells, and contribute to the activation of T cells through costimulatory molecule CD86 expression in inflamed colonic mucosa.

AB - Background and Aims: Costimulatory signals are essential for T-cell activation. The aim of this study was to demonstrate expression of costimulatory molecules CD86 and CD80 in human colonic epithelial cells and assess their functional roles in the activation of T cells in inflamed colonic mucosa. Methods: CD86 and CD80 expression was assessed by immunohistochemistry of colonic mucosa, reverse-transcription polymerase chain reaction, and flow cytometric analysis of isolated colonic epithelial cells and cell lines. Costimulatory effect of CD86-expressing colonic epithelial cells on purified CD4+ T-cell proliferation was also assessed at suboptimal phytohemagglutinin stimulation. Results: CD86 and CD80 messenger RNA was detected in isolated colonic epithelial cells from normal and inflamed mucosa of patients with ulcerative colitis. Immunohistochemistry and flow cytometric analysis of colonic epithelial cells confirmed cell surface expression of CD86 protein in inflamed colonic mucosa. Cell surface expression of CD86 protein was increased in the colonic epithelial cell line HT29-18-N2 after interferon gamma stimulation. Purified CD4+ T cells proliferated in response to suboptimal phytohemagglutinin costimulated with interferon gamma-stimulated HT2918-N2, and these proliferative responses were efficiently inhibited by the addition of anti-CD86 monoclonal antibody. Costimulatory effect of CD86-expressing colonic epithelial cells isolated from inflamed mucosa of ulcerative colitis was also demonstrated at suboptimal phytohemagglutinin stimulation in CD4+ T cells. Conclusions: Colonic epithelial cells may act as antigen-presenting cells, and contribute to the activation of T cells through costimulatory molecule CD86 expression in inflamed colonic mucosa.

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