Functional expressions of fms and M-CSF during trophoectodermal differentiation of human embryonal carcinoma cells

Nao Suzuki, Taketo Yamada, Kentaroh Matsuoka, Nobuyoshi Hiraoka, Yuji Iwamaru, Jun ichi Hata

Research output: Contribution to journalArticle

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Abstract

A human embryonal carcinoma (EC) cell line, NCR-G3 (G3), is capable of differentiating into a variety of cell types in vitro, including epithelial, muscle, neural and trophoectodermal cells. The production of human chorionic gonadotropin (hCG), a trophoectoderm-specific hormone, begins 7 days after retinoic acid (RA) treatment and peaks on day 12-13. In this study, we used G3 cells to investigate the biological significance of macrophage colony-stimulating factor (M-CSF), also called colony-stimulating factor-1 (CSF-1), and fms, a receptor tyrosine kinase for M-CSF. The mRNA of c-fms is constitutively expressed in both undifferentiated and differentiated G3 cells. Immunoprecipitation with anti-fms antibodies or flow cytometry revealed that differentiated G3 cells express fms on the cell surface. However, we were unable to demonstrate expression of fms on the surface of undifferentiated G3 cells. Expression of M-CSF mRNA and protein, however, was upregulated by RA treatment prior to hCG production. In order to investigate whether expression of both molecules is biologically functional in G3 cells, we conducted experiments using anti-M-CSF and fms antibodies with neutralizing activity and gene transfer to achieve over-expression of fms in G3 cells. As a result, we observed that hCG production following treatment with both neutralizing antibodies was more than 90 per cent inhibited, and that hCG production increased significantly as a result of over-expression of fms in G3 cells. Our results enabled us to show that M-CSF and fms play important functional roles in the differentiation of G3 cells into trophoectoderm. G3 cells are well suited to serve as an experimental model of human early embryogenesis and of placental differentiation.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalPlacenta
Volume20
Issue number2-3
DOIs
Publication statusPublished - 1999 Mar

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Embryonal Carcinoma Stem Cells
Macrophage Colony-Stimulating Factor
Chorionic Gonadotropin
Tretinoin
Neutralizing Antibodies
Messenger RNA
Receptor Protein-Tyrosine Kinases
Immunoprecipitation
Embryonic Development
Cell Differentiation
Anti-Idiotypic Antibodies
Flow Cytometry
Theoretical Models

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Suzuki, N., Yamada, T., Matsuoka, K., Hiraoka, N., Iwamaru, Y., & Hata, J. I. (1999). Functional expressions of fms and M-CSF during trophoectodermal differentiation of human embryonal carcinoma cells. Placenta, 20(2-3), 203-211. https://doi.org/10.1053/plac.1998.0367

Functional expressions of fms and M-CSF during trophoectodermal differentiation of human embryonal carcinoma cells. / Suzuki, Nao; Yamada, Taketo; Matsuoka, Kentaroh; Hiraoka, Nobuyoshi; Iwamaru, Yuji; Hata, Jun ichi.

In: Placenta, Vol. 20, No. 2-3, 03.1999, p. 203-211.

Research output: Contribution to journalArticle

Suzuki, N, Yamada, T, Matsuoka, K, Hiraoka, N, Iwamaru, Y & Hata, JI 1999, 'Functional expressions of fms and M-CSF during trophoectodermal differentiation of human embryonal carcinoma cells', Placenta, vol. 20, no. 2-3, pp. 203-211. https://doi.org/10.1053/plac.1998.0367
Suzuki, Nao ; Yamada, Taketo ; Matsuoka, Kentaroh ; Hiraoka, Nobuyoshi ; Iwamaru, Yuji ; Hata, Jun ichi. / Functional expressions of fms and M-CSF during trophoectodermal differentiation of human embryonal carcinoma cells. In: Placenta. 1999 ; Vol. 20, No. 2-3. pp. 203-211.
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