Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

Kwang Wook Ko, Fengqiu Lin, Takumi Katsumata, Yoshinori Sugai, Sho Miyazaki, Hiroshi Kawaide, Kazunori Okada, Hideaki Nojiri, Hisakazu Yamane

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

Original languageEnglish
Pages (from-to)3285-3288
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number12
DOIs
Publication statusPublished - 2008 Dec 1
Externally publishedYes

Fingerprint

Kaurane Diterpenes
Gibberellins
Pichia
Biosynthesis
Microsomes
Mixed Function Oxygenases
Yeast
Cytochrome P-450 Enzyme System
Oxidoreductases
Yeasts
Oxidation
ent-kaurene oxidase
kaurenoic acid
Oryza

Keywords

  • Cytochrome P450 monooxygenase (P450)
  • Ent-kaurene oxidase
  • Rice

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system. / Ko, Kwang Wook; Lin, Fengqiu; Katsumata, Takumi; Sugai, Yoshinori; Miyazaki, Sho; Kawaide, Hiroshi; Okada, Kazunori; Nojiri, Hideaki; Yamane, Hisakazu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 12, 01.12.2008, p. 3285-3288.

Research output: Contribution to journalArticle

Ko, KW, Lin, F, Katsumata, T, Sugai, Y, Miyazaki, S, Kawaide, H, Okada, K, Nojiri, H & Yamane, H 2008, 'Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system', Bioscience, Biotechnology and Biochemistry, vol. 72, no. 12, pp. 3285-3288. https://doi.org/10.1271/bbb.80427
Ko, Kwang Wook ; Lin, Fengqiu ; Katsumata, Takumi ; Sugai, Yoshinori ; Miyazaki, Sho ; Kawaide, Hiroshi ; Okada, Kazunori ; Nojiri, Hideaki ; Yamane, Hisakazu. / Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 12. pp. 3285-3288.
@article{bf0e1eb7610b4fe091658f1e60a4345a,
title = "Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system",
abstract = "Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.",
keywords = "Cytochrome P450 monooxygenase (P450), Ent-kaurene oxidase, Rice",
author = "Ko, {Kwang Wook} and Fengqiu Lin and Takumi Katsumata and Yoshinori Sugai and Sho Miyazaki and Hiroshi Kawaide and Kazunori Okada and Hideaki Nojiri and Hisakazu Yamane",
year = "2008",
month = "12",
day = "1",
doi = "10.1271/bbb.80427",
language = "English",
volume = "72",
pages = "3285--3288",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "12",

}

TY - JOUR

T1 - Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

AU - Ko, Kwang Wook

AU - Lin, Fengqiu

AU - Katsumata, Takumi

AU - Sugai, Yoshinori

AU - Miyazaki, Sho

AU - Kawaide, Hiroshi

AU - Okada, Kazunori

AU - Nojiri, Hideaki

AU - Yamane, Hisakazu

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

AB - Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

KW - Cytochrome P450 monooxygenase (P450)

KW - Ent-kaurene oxidase

KW - Rice

UR - http://www.scopus.com/inward/record.url?scp=58149086984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149086984&partnerID=8YFLogxK

U2 - 10.1271/bbb.80427

DO - 10.1271/bbb.80427

M3 - Article

C2 - 19060383

AN - SCOPUS:58149086984

VL - 72

SP - 3285

EP - 3288

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 12

ER -