Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system

Kwang Wook Ko, Fengqiu Lin, Takumi Katsumata, Yoshinori Sugai, Sho Miyazaki, Hiroshi Kawaide, Kazunori Okada, Hideaki Nojiri, Hisakazu Yamane

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

Original languageEnglish
Pages (from-to)3285-3288
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number12
DOIs
Publication statusPublished - 2008 Dec 1
Externally publishedYes

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Keywords

  • Cytochrome P450 monooxygenase (P450)
  • Ent-kaurene oxidase
  • Rice

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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