Functional significance of stage-specific embryonic antigens in the development of preimplantation embryos

Yohko U. Katagiri; Ban Sato; Kenji Miyado; Hidenori Akutsu; Hajime Okita; Akihiro Umezawa; Junichiro Fujimoto; Nobutaka Kiyokawa

doi:10.4052/tigg.20.131

Functional significance of stage-specific embryonic antigens in the development of preimplantation embryos

Yohko U. Katagiri, Ban Sato, Kenji Miyado, Hidenori Akutsu, Hajime Okita, Akihiro Umezawa, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journal › Article › peer-review

Abstract

In preimplantation embryos, stage-specific embryonic antigen (SSEA)-1, SSEA-3, and SSEA-4 are well-known carbohydrate marker antigens that show a stage-specific expression. SSEA-1 is usually carried by glycoproteins and/or glycolipids, while SSEA-3 and SSEA-4 are mostly carried by glycosphingolipids, which are the major constituents of raft microdomains. Biochemical analysis and confocal laser-scanning microscopic study of raft microdomains have been conducted in cultured cell lines. Here, we show the dynamic movement of SSEA-4 in the raft microdomains of preimplantation mouse embryos by immunostaining with 6E2, a novel anti-SSEA-4 monoclonal antibody, and subsequently discuss the role of raft microdomains in early embryogenesis.

Original language	English
Pages (from-to)	131-139
Number of pages	9
Journal	Trends in Glycoscience and Glycotechnology
Volume	20
Issue number	113
DOIs	https://doi.org/10.4052/tigg.20.131
Publication status	Published - 2008 Jul
Externally published	Yes

Keywords

Preimplantation mouse embryo
Raft
SSEA
SialylGb5Cer

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.4052/tigg.20.131

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title = "Functional significance of stage-specific embryonic antigens in the development of preimplantation embryos",

abstract = "In preimplantation embryos, stage-specific embryonic antigen (SSEA)-1, SSEA-3, and SSEA-4 are well-known carbohydrate marker antigens that show a stage-specific expression. SSEA-1 is usually carried by glycoproteins and/or glycolipids, while SSEA-3 and SSEA-4 are mostly carried by glycosphingolipids, which are the major constituents of raft microdomains. Biochemical analysis and confocal laser-scanning microscopic study of raft microdomains have been conducted in cultured cell lines. Here, we show the dynamic movement of SSEA-4 in the raft microdomains of preimplantation mouse embryos by immunostaining with 6E2, a novel anti-SSEA-4 monoclonal antibody, and subsequently discuss the role of raft microdomains in early embryogenesis.",

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AU - Katagiri, Yohko U.

AU - Sato, Ban

AU - Miyado, Kenji

AU - Akutsu, Hidenori

AU - Okita, Hajime

AU - Umezawa, Akihiro

AU - Fujimoto, Junichiro

AU - Kiyokawa, Nobutaka

PY - 2008/7

Y1 - 2008/7

N2 - In preimplantation embryos, stage-specific embryonic antigen (SSEA)-1, SSEA-3, and SSEA-4 are well-known carbohydrate marker antigens that show a stage-specific expression. SSEA-1 is usually carried by glycoproteins and/or glycolipids, while SSEA-3 and SSEA-4 are mostly carried by glycosphingolipids, which are the major constituents of raft microdomains. Biochemical analysis and confocal laser-scanning microscopic study of raft microdomains have been conducted in cultured cell lines. Here, we show the dynamic movement of SSEA-4 in the raft microdomains of preimplantation mouse embryos by immunostaining with 6E2, a novel anti-SSEA-4 monoclonal antibody, and subsequently discuss the role of raft microdomains in early embryogenesis.

AB - In preimplantation embryos, stage-specific embryonic antigen (SSEA)-1, SSEA-3, and SSEA-4 are well-known carbohydrate marker antigens that show a stage-specific expression. SSEA-1 is usually carried by glycoproteins and/or glycolipids, while SSEA-3 and SSEA-4 are mostly carried by glycosphingolipids, which are the major constituents of raft microdomains. Biochemical analysis and confocal laser-scanning microscopic study of raft microdomains have been conducted in cultured cell lines. Here, we show the dynamic movement of SSEA-4 in the raft microdomains of preimplantation mouse embryos by immunostaining with 6E2, a novel anti-SSEA-4 monoclonal antibody, and subsequently discuss the role of raft microdomains in early embryogenesis.

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KW - Raft

KW - SSEA

KW - SialylGb5Cer

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Functional significance of stage-specific embryonic antigens in the development of preimplantation embryos

Abstract

Keywords

ASJC Scopus subject areas

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