Functional significance of stage-specific embryonic antigens in the development of preimplantation embryos

Yohko U. Katagiri, Ban Sato, Kenji Miyado, Hidenori Akutsu, Hajime Okita, Akihiro Umezawa, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journalArticle

Abstract

In preimplantation embryos, stage-specific embryonic antigen (SSEA)-1, SSEA-3, and SSEA-4 are well-known carbohydrate marker antigens that show a stage-specific expression. SSEA-1 is usually carried by glycoproteins and/or glycolipids, while SSEA-3 and SSEA-4 are mostly carried by glycosphingolipids, which are the major constituents of raft microdomains. Biochemical analysis and confocal laser-scanning microscopic study of raft microdomains have been conducted in cultured cell lines. Here, we show the dynamic movement of SSEA-4 in the raft microdomains of preimplantation mouse embryos by immunostaining with 6E2, a novel anti-SSEA-4 monoclonal antibody, and subsequently discuss the role of raft microdomains in early embryogenesis.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalTrends in Glycoscience and Glycotechnology
Volume20
Issue number113
DOIs
Publication statusPublished - 2008 Jul 1

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Keywords

  • Preimplantation mouse embryo
  • Raft
  • SSEA
  • SialylGb5Cer

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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